Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Resource
  • Published:

Multiplex Fluorescent Immunoassay for the simultaneous detection of serum antibodies to multiple rodent pathogens

Lab Animal volume 36, pages 36–38 (2007)Cite this article

Abstract

Multiplex Fluorescent Immunoassay (MFI) is a sensitive and specific serologic test that allows simultaneous detection of antibodies to multiple viral and bacterial agents in a single reaction well. MFI is a high-throughput assay that offers several advantages over other prevalent assays, and some research animal diagnostic laboratories have adopted it as their primary technique. The authors present a detailed review of MFI and its application to laboratory animal diagnostics.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Protein antigens from different infectious disease agents are covalently coupled to uniquely color-coded microspheres in separate tubes.
Figure 2
Figure 3: The test serum sample is added to the reaction well containing the antigen-coupled microspheres.
Figure 4: Secondary antibodies to immunoglobulin labeled with phycoerythrin (PE) are added to the reaction well.
Figure 5: Microspheres are analyzed with a specialized flow cytometer with two lasers.

Similar content being viewed by others

References

  1. Hsu, C.C., Wobus, C.E., Steffen, E.K., Riley, L.K. & Livingston, R.S. Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice. Clin. Diagn. Lab. Immunol. 12, 1145–1151 (2005).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Khan, I.H. et al. Simultaneous serodetection of 10 highly prevalent mouse infectious pathogens in a single reaction by multiplex analysis. Clin. Diagn. Lab. Immunol. 12, 513–519 (2005).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Khan, S.S., Smith, M.S., Reda, D., Suffredini, A.F. & McCoy, J.P. Jr. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers. Cytometry B Clin. Cytom. 61, 35–39 (2004).

    Article  Google Scholar 

  4. Hsu, C.C., Riley, L.K., Wills, H.M. & Livingston, R.S. Persistent infection and serologic cross-reactivity of three novel murine noroviruses. Comp. Med. 56, 247–251 (2006).

    CAS  PubMed  Google Scholar 

  5. Biagini, R.E. et al. Comparison of a multiplexed fluorescent covalent microsphere immunoassay and an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins. Clin. Diagn. Lab. Immunol. 11, 50–55 (2004).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Kendall, L.V. & Riley, L.K. Enzyme-Linked Immunosorbent Assay (ELISA). Contemp. Top. Lab. Anim. Sci. 38, 47 (1999).

    PubMed  Google Scholar 

  7. Kendall, L.V., Steffen, E.K. & Riley, L.K. Indirect Fluorescent Antibody (IFA) Assay. Contemp. Top. Lab. Anim. Sci. 38, 68 (1999).

    PubMed  Google Scholar 

  8. Kendall, L.V., Steffen, E.K. & Riley, L.K. Hemagglutination Inhibition (HAI) Assay. Contemp. Top. Lab. Anim. Sci. 38, 54 (1999).

    PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank Howard Wilson and Don Connor for their technical and graphical assistance with the figures.

Author information

Authors and Affiliations

  1. Department of Veterinary Pathobiology, Research Animal Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO

    Charlie C. Hsu,  Craig Franklin &  Lela K. Riley

Authors
  1. Charlie C. Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Craig Franklin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lela K. Riley
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Charlie C. Hsu.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hsu, C., Franklin, C. & Riley, L. Multiplex Fluorescent Immunoassay for the simultaneous detection of serum antibodies to multiple rodent pathogens. Lab Anim 36, 36–38 (2007). https://doi.org/10.1038/laban0907-36

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/laban0907-36

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing