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Reproduction and fertility in wild-type and transgenic mice after orthotopic transplantation of cryopreserved ovaries from 10-d-old mice

Lab Animal volume 37, pages 353–357 (2008)Cite this article

Abstract

Ovary cryopreservation and subsequent transplantation can enable researchers to preserve valuable transgenic animal strains. Some studies have indicated, however, that this process may impair ovary viability and recipient fertility. The authors investigated the effects of ovary vitrification followed by orthotopic transplantation in five strains of mice. They grafted fresh and frozen ovaries of 10-d-old mice into 4-week-old ovariectomized recipients. In addition to using wild-type strains (BALB/cAn and ICR/JCL), the authors used a transgene system that enabled them to identify whether offspring derived from the ovary of the recipient or that of the donor: they transplanted ovaries from one transgenic strain (LAP/rtTA) into wild-type C57BL/6J mice and into mice from a second transgenic strain (pTet/Cd226). The authors then determined the origin of the offspring born to these recipients using PCR. Ovary cryopreservation seemed to have no effect on the long-term fertility and reproductive characteristics of recipients and their offspring.

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Acknowledgements

This study was supported by the Science and Technology Commission of Shanghai Municipality (serial number 054909007) and by the National Key Technology R&D Program of China (grant number 2006BAI23B04). We also thank Zong-Guo-Sun for help in running the PCR test.

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Authors and Affiliations

  1. Shanghai Laboratory Animal Center, Chinese Academy of Sinica, Shanghai, 201615, China

    Li-Jun Liu,  Xia-Yang Xie,  Rui-Zhong Zhang &  Ping Xu

  2. Zentrum fuer Molekulare Biologie der Universitaet Heidelberg, Im Neuenheimer Feld 282, Heidelberg, D-69120, Germany

    Hermann Bujard

  3. Department of Etiology, Fourth Military Medical University, Xi'an, 710032, China

    Miao Jun

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Correspondence to Ping Xu.

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Liu, LJ., Xie, XY., Zhang, RZ. et al. Reproduction and fertility in wild-type and transgenic mice after orthotopic transplantation of cryopreserved ovaries from 10-d-old mice. Lab Anim 37, 353–357 (2008). https://doi.org/10.1038/laban0808-353

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