Abstract
The production of recombinant proteins in the milk of transgenic animals is an alternative to traditional cell culture methodology. Transgenic rabbits can serve in the small-scale production of recombinant proteins, underscoring the need to maintain valuable transgenic lines. In this study, the authors used cryopreserved transgenic rabbit semen to artificially inseminate does, demonstrating the utility of this method for the reestablishment of a transgenic rabbit herd.
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References
Hammer, R.E. et al. Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315(6021), 681–683 (1985).
Meade, H.M. et al. in Gene Expression Systems: Using Nature for the Art of Expression (eds Fernandez, J.M. & Hoeffler, J.P.) 400–421 (Academic Press, San Diego, 1999).
Pollock, D.P. et al. Transgenic milk as a method for the production of recombinant antibodies. J. Immunol. Methods 231(1–2), 147–157 (1999).
Ziomek, C.A. Commercialization of protein produced in the mammary gland. Theriogenology 49(1), 139–144 (1998).
Vicente, J.S. & Viudes-de-Castro, M.P. A sucrose-DMSO extender for freezing rabbit semen. Reprod. Nutr. Dev. 36(5), 485–492 (1995).
Wales, R.G. & O'Shea, T. The deep freezing of rabbit spermatozoa. Aust. J. Biol. Sci. 21(4), 831–833 (1968).
FDA Center for Biologics Evaluation and Research. Points to Consider in the Manufacture and Testing of Therapeutic Products for Human Use Derived from Transgenic Animals. (1995). http://www.fda.gov/cber/gdlns/ptc_tga.txt.
Gavin, W. The future of transgenics. Regulatory Affairs Focus 6, 13–19 (2001).
Baguisi, A. et al. Production of goats by somatic cell transfer. Nat. Biotechnol. 17(5), 456–461 (1999).
Dalimata, A.M. & Graham, J.K. Cryopreservation of rabbit spermatozoa using acetamide in combination with trehalose and methyl cellulose. Theriogenology 48(5), 831–841 (1997).
Blash, S., Melican, D. & Gavin, W. Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology 54(6), 899–905 (2000).
Acknowledgements
The authors gratefully acknowledge the help and hard work of the Genzyme LARD staff, especially Joanne Fagan, Debbie Dimke, and Stephen Baker. We wish to thank Dr. Carol Ziomek and David Melican of GTC Biotherapeutics for their support and for critical review of this manuscript.
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Blash, S., Chen, L., Harvey, M. et al. Reestablishment of a transgenic rabbit line by artificial insemination using cryopreserved semen. Lab Anim 34, 61–63 (2005). https://doi.org/10.1038/laban0405-61
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DOI: https://doi.org/10.1038/laban0405-61
