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Archiving mouse strains by cryopreservation

Lab Animal volume 34, pages 50–57 (2005)Cite this article

Abstract

A great deal of time and energy goes into the creation of each new line of transgenic mice; established lines are expensive and labor-intensive to maintain. Archiving of mice by cryopreservation of germ cells or embryos represents a means to free up facility space, while protecting the line from loss due to environmental disasters, genetic drift, or infectious disease. The author reviews the available cryopreservation techniques and presents considerations for setting up a cryopreservation facility.

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Figure 1: Fixation of new mutations during maintenance breeding of an inbred strain.

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References

  1. Mobraaten, L.E. Mouse embryo cryobanking. J. In Vitro Fert. Embryo Transf. 3(1), 28–32 (1986).

    Article  Google Scholar 

  2. Mobraaten, L. Cryopreservation in a transgenic program. Lab Anim. (NY) 28(Suppl 1), 15–18 (1999).

    Google Scholar 

  3. Glenister, P.H. & Thornton, C.E. Cryoconservation—archiving for the future. Mamm. Genome 11(7), 565–571 (2000).

    Article  Google Scholar 

  4. Critser, J.K. & Mobraaten, L.E. Cryopreservation of murine spermatozoa. ILAR J. 41(4), 197–206 (2000).

    Article  Google Scholar 

  5. Marschall, S. & Hrabe de Angelis, M. Cryopreservation of mouse spermatozoa: double your mouse space. Trends Genet. 15(4), 128–131 (1999).

    Article  Google Scholar 

  6. Whittingham, D.G., Leibo, S.P. & Mazur, P. Survival of mouse embryos frozen to −196 degrees and −269 degrees C. Science 178(59), 411–414 (1972).

    Article  Google Scholar 

  7. Leibo, S.P. Personal communication. Dr. Leibo elaborates, “That remark was made to me by a very close friend [at the Basel Institute for Immunology], in about 1974 or 75.”

  8. Culliton, B.J. Fire devastates Jackson lab. Science 244(4906), 767–768 (1989).

    Google Scholar 

  9. Sincell, M. Houston flood. Research toll is heavy in time and money. Science 293(5530), 589 (2001).

    Article  Google Scholar 

  10. Nakagata, N. et al. Cryopreservation of spermatozoa of a transgenic mouse. [Japanese] Jikken Dobutsu 41(4), 537–540 (1992).

    Google Scholar 

  11. Nakagata, N. & Takeshima, T. Cryopreservation of mouse spermatozoa from inbred and F1 hybrid strains. Jikken Dobutsu 42(3), 317–320 (1993).

    Google Scholar 

  12. Nakagata, N. Studies on cryopreservation of embryos and gametes in mice. Exp. Anim. 44(1), 1–8 (1995).

    Article  Google Scholar 

  13. Nakagata, N. Use of cryopreservation techniques of embryos and spermatozoa for production of transgenic (Tg) mice and for maintenance of Tg mouse lines. Lab. Anim. Sci. 46(2), 236–238 (1996).

    Google Scholar 

  14. Nakagata, N. Cryopreservation of mouse spermatozoa. Mamm. Genome 11(7), 572–576 (2000).

    Article  Google Scholar 

  15. Sztein, J., Sweet, H., Farley, J. & Mobraaten, L. Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking. Biol. Reprod. 58(4), 1071–1074 (1998).

    Article  Google Scholar 

  16. Migishima, F. et al. Successful cryopreservation of mouse ovaries by vitrification. Biol. Reprod. 68(3), 881–887 (2003).

    Article  Google Scholar 

  17. Nakao, K., Nakagata, N. & Katsuki, M. Simple and efficient vitrification procedure for cryopreservation of mouse embryos. Exp. Anim. 46(3), 231–234 (1997).

    Article  Google Scholar 

  18. Chen, S.U. et al. Vitrification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws (OPS) and grids. Hum. Reprod. 16(11), 2350–2356 (2001).

    Article  Google Scholar 

  19. Park, S.E. et al. Cryopreservation of ICR mouse oocytes: improved post-thawed preimplantation development after vitrification using Taxol, a cytoskeleton stabilizer. Fertil. Steril. 75(6), 1177– 1184 (2001).

    Article  Google Scholar 

  20. Lane, M. & Gardner, D.K. Vitrification of mouse oocytes using a nylon loop. Mol. Reprod. Dev. 58(3), 342–347 (2001).

    Article  Google Scholar 

  21. Avarbock, M.R., Brinster, C.J. & Brinster, R.L. Reconstitution of spermatogenesis from frozen spermatogonial stem cells. Nat. Med. 2(6), 693–696 (1996).

    Article  Google Scholar 

  22. Rall, W.F. & Fahy, G.M. Ice-free cryopreservation of mouse embryos at −196 °C by vitrification. Nature 313(6003), 573–575 (1985).

    Article  Google Scholar 

  23. Sztein, J.M., Farley, J.S. & Mobraaten, L.E. In vitro fertilization with cryopreserved inbred mouse sperm. Biol. Reprod. 63(6), 1774–1780 (2000).

    Article  Google Scholar 

  24. Kimura, Y. & Yanagimachi, R. Intracytoplasmic sperm injection in the mouse. Biol. Reprod. 52(4), 709–720 (1995).

    Article  Google Scholar 

  25. Lacham-Kaplan, O. & Trounson, A. Intracytoplasmic sperm injection in mice: increased fertilization and development to term after induction of the acrosome reaction. Hum. Reprod. 10(10), 2642–2649 (1995).

    Article  Google Scholar 

  26. Yanagimachi, R. Intracytoplasmic sperm injection experiments using the mouse as a model. Hum. Reprod. 13(Suppl 1), 87–98 (1998).

    Article  Google Scholar 

  27. Szczygiel, M.A., Kusakabe, H., Yanagimachi, R. & Whittingham, D.G. Intracytoplasmic sperm injection is more efficient than in vitro fertilization for generating mouse embryos from cryopreserved spermatozoa. Biol. Reprod. 67(4), 1278–1284 (2002).

    Article  Google Scholar 

  28. Nakagata, N., Okamoto, M., Ueda, O. & Suzuki, H. Positive effect of partial zona-pellucida dissection on the in vitro fertilizing capacity of cryopreserved C57BL/6J transgenic mouse spermatozoa of low motility. Biol. Reprod. 57(5), 1050–1055 (1997).

    Article  Google Scholar 

  29. Nagy, A., Gertsenstein, M., Vintersten, K. & Behringer, R. Manipulating the Mouse Embryo: A Laboratory Manual 3rd edn. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2003).

    Google Scholar 

  30. Dufrain, R.J. in Basic Aspects of Freeze-Preservation of Mouse Strains (ed. Mühlbock, O.) 73–84 (Gustav Fischer, Stuttgart, 1976).

    Google Scholar 

  31. Mazur, P. in Basic Aspects of Freeze-Preservation of Mouse Strains (ed. Mühlbock, O.) 1–12 (Gustav Fischer, Stuttgart, 1976).

    Google Scholar 

  32. Hawkins, A.E. et al. Hepatitis B nucleotide sequence analysis: linking an outbreak of acute hepatitis B to contamination of a cryopreservation tank. J. Virol. Methods 60(1), 81–88 (1996).

    Article  Google Scholar 

  33. Tedder, R.S. et al. Hepatitis B transmission from contaminated cryopreservation tank. Lancet 346(8968), 137–140 (1995).

    Article  Google Scholar 

  34. Van Keuren, M.L. & Saunders, T.L. Rederivation of transgenic and gene-targeted mice by embryo transfer. Transgenic Res. 13(4), 363–371 (2004).

    Article  Google Scholar 

  35. Suzuki, H., Yorozu, K., Watanabe, T., Nakura, M. & Adachi, J. Rederivation of mice by means of in vitro fertilization and embryo transfer. Exp. Anim. 45(1), 33–38 (1996).

    Article  Google Scholar 

  36. Reetz, I.C., Wullenweber-Schmidt, M., Kraft, V. & Hedrich, H.J. Rederivation of inbred strains of mice by means of embryo transfer. Lab. Anim. Sci. 38(6), 696–701 (1988).

    Google Scholar 

  37. Carthew, P., Wood, M.J. & Kirby, C. Pathogenicity of mouse hepatitis virus for preimplantation mouse embryos. J. Reprod. Fertil. 73(1), 207–213 (1985).

    Article  Google Scholar 

  38. Morrell, J.M. Techniques of embryo transfer and facility decontamination used to improve the health and welfare of transgenic mice. Lab. Anim. 33(3), 201–206 (1999).

    Article  Google Scholar 

  39. Legge, M. Oocyte and zygote zona pellucida permeability to macromolecules. J. Exp. Zool. 271(2), 145–150 (1995).

    Article  Google Scholar 

  40. Baillargeon, P., Fecteau, G., Pare, J., Lamothe, P. & Suave, R. Evaluation of the embryo transfer procedure proposed by the International Embryo Transfer Society as a method of controlling vertical transmission of Neospora caninum in cattle. J. Am. Vet. Med. Assoc. 218(11), 1803–1806 (2001).

    Article  Google Scholar 

  41. Stringfellow, D.A. & Seidel, S.M. IETS Manual 3rd edn (International Embryo Transfer Society, Sazvoy, IL, 1998).

    Google Scholar 

  42. Leibo, S.P., DeMayo, F.J. & O'Malley, B. Production of transgenic mice from cryopreserved ova. Mol. Reprod. Dev. 30(4), 313–319 (1991).

    Article  Google Scholar 

  43. Landa, V. & Slezinger, M.S. Production of transgenic mice from DNA-injected embryos cryopreserved by vitrification in microdrops. Folia Biol. (Praha) 38(1), 10–15 (1992).

    Google Scholar 

  44. Keskintepe, L. Agca, Y., Pacholczyk, G.A., Machnicka, A. & Critser, J.K. Use of cryopreserved pronuclear embryos for the production of transgenic mice. Biol. Reprod. 65(2), 407–411 (2001).

    Article  Google Scholar 

  45. Bagis, H., Odaman, H., Sagirkaya, H. & Dinnyes, A. Production of transgenic mice from vitrified pronuclear-stage embryos. Mol. Reprod. Dev. 61(2), 173–179 (2002).

    Article  Google Scholar 

  46. Mazur, P., Katkov, II, Katkova, N. & Critser, J.K. The enhancement of the ability of mouse sperm to survive freezing and thawing by the use of high concentrations of glycerol and the presence of an Escherichia coli membrane preparation (Oxyrase) to lower the oxygen concentration. Cryobiology 40(3), 187–209 (2000).

    Article  Google Scholar 

  47. Koshimoto, C., Gamliel, E. & Mazur, P. Effect of osmolality and oxygen tension on the survival of mouse sperm frozen to various temperatures in various concentrations of glycerol and raffinose. Cryobiology 41(3), 204–231 (2000).

    Article  Google Scholar 

  48. Koshimoto, C. & Mazur, P. The effect of the osmolality of sugar-containing media, the type of sugar, and the mass and molar concentration of sugar on the survival of frozen-thawed mouse sperm. Cryobiology 45(1), 80–90 (2002).

    Article  Google Scholar 

  49. Koshimoto, C. & Mazur, P. Effects of warming rate, temperature, and antifreeze proteins on the survival of mouse spermatozoa frozen at an optimal rate Cryobiology 45(1), 49–59 (2002).

    Article  Google Scholar 

  50. Bath, M.L. Simple and efficient in vitro fertilization with cryopreserved C57BL/6J mouse sperm. Biol. Reprod. 68(1), 19–23 (2003).

    Article  Google Scholar 

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Author notes

  1. Carlisle P. Landel PhD

    Present address: Present address: Transgenic and Gene Targeting Facility, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107.,

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  1. Cryopreservation Laboratory, The Jackson Laboratory, Bar Harbor, 04679, ME

    Carlisle P. Landel PhD

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  1. Carlisle P. Landel PhD
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Correspondence to Carlisle P. Landel PhD.

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Landel, C. Archiving mouse strains by cryopreservation. Lab Anim 34, 50–57 (2005). https://doi.org/10.1038/laban0405-50

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  • DOI: https://doi.org/10.1038/laban0405-50

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