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Use of the i-STAT portable clinical analyzer in mice

Lab Animal volume 35, pages 45–50 (2006)Cite this article

Abstract

The use of portable chemistry analyzers is an attractive option for obtaining clinical pathology panels in mice, because these analyzers require only small volumes of whole blood. However, in studies with other animals, portable analyzers do not always agree with results obtained using standard laboratory equipment. The authors evaluated the use of the i-STAT handheld portable clinical analyzer compared to the use of standard nonportable laboratory instruments in mice. As shown with other species, the i-STAT results did not always agree with standard laboratory instruments; however, the i-STAT does show reliability for certain chemistry assays.

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Figure 1: Bland-Altman plot comparing differences in hemoglobin values between the i-STAT analyzer and the Abbott Cell-Dyn 3700 CS hematology analyzer.
Figure 2: Scatterplot of mean hemoglobin values of paired samples versus the differences in values between the two methods (i-STAT − CellDyne).

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References

  1. Jacoby, R.O., Fox, J.G. & Davisson, M.T. in Laboratory Animal Medicine (eds. Fox, J.G., Anderson, L.C., Loew, F.M. & Quimby, F.W.) 35–120 (Academic Press, New York, 2002).

    Book  Google Scholar 

  2. Diehl, K.H. et al. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J. Appl. Toxicol. 21(1), 15–23 (2001).

    Article  Google Scholar 

  3. Anonymous. Removal of blood from laboratory mammals and birds. First report of the BVA/FRAME/RSPCA/UFAW Joint Working Group on Refinement. Lab. Anim. 27(1), 1–22 (1993).

  4. Hoff, J. Methods of blood collection in the mouse. Lab Anim. (NY) 29(10), 47–53 (2000).

    Google Scholar 

  5. Erickson, K.A. & Wilding, P. Evalution of a novel point-of-care system, the i-Stat Portable Clinical Analyzer. Clin. Chem. 39(2), 283–287 (1993).

    Google Scholar 

  6. Jacobs, E. Vadasdi, E., Sarkozi, L. & Colman, N. Analytical evaluation of i-Stat Portable Clinical Analyzer and use by nonlaboratory health-care professionals. Clin. Chem. 39(6), 1069–1074 (1993).

    Google Scholar 

  7. Kisner, H.J. i-Stat point-of-care blood analyzing system. Clin. Lab. Manage. Rev. 7(5), 454–457 (1993).

    Google Scholar 

  8. Sediame, S., Zerah-Lancner, F., d'Ortho, M.P., Adnot, S. & Harf, A. Accuracy of the I-Stat bedside blood gas analyzer. Eur. Respir. J. 14(1), 214–217 (1999).

    Article  Google Scholar 

  9. Cohn, L.A., McCaw, D.L., Tate, D.J. & Johnson, J.C. Assessment of five portable blood glucose meters, a point-of-care analyzer, and color test strips for measuring blood glucose concentration in dogs. J. Am. Vet. Med. Assoc. 216(2), 198–202 (2000).

    Article  Google Scholar 

  10. Grosenbaugh, D.A., Gadawski, J.E. & Muir, W.W. Evaluation of a portable clinical analyzer in a veterinary hospital setting. J. Am. Vet. Med. Assoc. 213(5), 691–694 (1998).

    Google Scholar 

  11. Larsen, R.S., Haulena, M., Grindem, C.B. & Gulland, F.M. Blood values of juvenile northern elephant seals (Mirounga angustirostris) obtained using a portable clinical analyzer. Vet. Clin. Pathol. 31(3), 106–110 (2002).

    Article  Google Scholar 

  12. Looney, A.L., Ludders, J., Erb, H.N., Gleed, R. & Moon, P. Use of a handheld device for analysis of blood electrolyte concentrations and blood gas partial pressures in dogs and horses. J. Am. Vet. Med. Assoc. 213(4), 526–530 (1998).

    Google Scholar 

  13. Verwaerde, P., Malet, C., Lagente, M., de la Farge, F. & Braun, J.P. The accuracy of the i-Stat Portable Analyser for measuring blood gases and pH in whole-blood samples from dogs. Res. Vet. Sci. 43(1), 71–75 (2002).

    Article  Google Scholar 

  14. Bland, J.M. & Altman, D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1(8476), 307–310 (1986).

    Article  Google Scholar 

  15. Gray, T.E., Pratt, M.C. & Cusick, P.K. Determination of agreement between laboratory instruments. Contemp. Top. Lab. Anim. Sci. 38(2), 56–59 (1999).

    Google Scholar 

  16. Pinckard, J.K., Zahn, J., Ashby, L., Parvin, C.A. & Scott, M.G. Falsely increased i-Stat chloride results for blood samples with increased urea. Clin. Chem. 47(11), 2064–2066 (2001).

    Google Scholar 

  17. National Committee for Laboratory Standards. Procedures for the Handling and Processing of Blood Specimens: Approved Guideline 3rd edn. (Clinical and Laboratory Standards Institute, Wayne, PA, 2004).

Download references

Acknowledgements

The authors thank Helen Jackson and the staff of the Section of Veterinary Laboratory Medicine for technical assistance, Lianchun Xiao of the Department of Biostatistics and Applied Mathematics for assistance with statistical analyses, and Dr. Kenneth N. Gray of the Department of Veterinary Medicine and Surgery at the University of Texas M.D. Anderson Cancer Center for providing financial support for this study.

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  1. Department of Veterinary Medicine and Surgery, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, 77030, TX

    Peggy Tinkey DVM, Thomas Lembo DVM, Suzanne Craig DVM, Cheri West DVM & Carolyn Van Pelt DVM, PhD

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  1. Peggy Tinkey DVM
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  2. Thomas Lembo DVM
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  3. Suzanne Craig DVM
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Correspondence to Peggy Tinkey DVM.

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The authors declare no competing financial interests.

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Tinkey, P., Lembo, T., Craig, S. et al. Use of the i-STAT portable clinical analyzer in mice. Lab Anim 35, 45–50 (2006). https://doi.org/10.1038/laban0206-45

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