Abstract
Hsp70 is often overexpressed in cancer cells, and the selective cellular survival advantage that it confers may contribute to the process of tumour formation. Thus, the pharmacological manipulation of Hsp70 levels in cancer cells may be an effective means of preventing the progression of tumours. We found that the downregulation of Hsp70 by ibuprofen in vitro enhances the antitumoural activity of cisplatin in lung cancer. Ibuprofen prominently suppressed the expression of Hsp70 in A549 cells derived from lung adenocarcinoma and sensitized them to cisplatin in association with an increase in the mitochondrial apoptotic cascade, whereas ibuprofen alone did not induce cell death. The cisplatin-dependent events occurring up- and downstream of mitochondrial disruption were accelerated by treatment with ibuprofen. The increase in cisplatin-induced apoptosis caused by the depletion of Hsp70 by RNA interference is evidence that the increased apoptosis by ibuprofen is mediated by its effect on Hsp70. Our observations indicate that the suppression of Hsp70 by ibuprofen mediates the sensitivity to cisplatin by enhancing apoptosis at several stages of the mitochondrial cascade. Ibuprofen, therefore, is a potential therapeutic agent that might allow lowering the doses of cisplatin and limiting the many challenge associated with its toxicity and development of drug resistance.
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Main
The human Hsp70 family includes ≥8 highly homologous members that differ from each other by their intracellular localization and expression patterns.1 Among them, the major stress-inducible Hsp70 (also called Hsp72) has an essential role in cell survival under stressful conditions. Compared with its normal counterpart, Hsp70 is often overexpressed in various cancer cells and is suspected to contribute to the development of tumours.2, 3 Indeed, the expression of Hsp70 in certain cancer types has been correlated with poor prognosis and resistance to chemotherapy.4, 5, 6
Tumour cells often express several proteins that, when abnormally elevated, render the tumour resistant to apoptosis.7 Previous studies have confirmed not only that Hsp70 is cytoprotective, but also that it interferes effectively with cell death induced by a wide variety of stimuli, including several cancer-related stresses. Hsp70 is a potent inhibitor of the stress-activated kinase pathway, and apparently blocks apoptotic signals via interactions with JNK, Ask1 and SEK1.8, 9, 10, 11 Hsp70 is also a negative regulator of the mitochondrial pathway of apoptosis. Much of the focus on the antiapoptotic function of Hsp70 has been on events that occur after the disruption of the mitochondria. Hsp70 prevents the recruitment of procaspase-9 to the apoptosome, and its functional complex formation by direct interaction with apoptotic protease-activating factor 1 (Apaf-1).12, 13 Furthermore, Hsp70 inhibits the activation of caspase-3 and the cleavage of caspase-3 targets, such as ICAD and GATA-1.14, 15 On the other hand, recent studies have reported that Hsp70 can prevent apoptosis upstream of the mitochondria, by inhibiting events, which, ultimately, permeabilize the mitochondrial outer membrane, such as the activation of Bax.16, 17
As a result of the inhibition by Hsp70 of the apoptosis induced by several anticancer drugs, as well as by other stimuli, we hypothesized that cancer cells would be sensitized to the induction of apoptosis by the neutralization of Hsp70. Hsp70 has been, indeed, targeted with pharmaceuticals, such as triptolide, quercetin and KNK437, which downregulate its expression.18, 19, 20 Although they have prevented the progression of various cancer cells in vitro and in vivo,21, 22 the optimal clinical use of these small Hsp70 inhibitors singly or combined with other chemotherapeutics remains a challenge. Our overall objective was to pharmacologically control the levels of Hsp70, and increase the effectiveness of anticancer drugs.
Several experimental and epidemiologic studies and clinical trials have observed a powerful chemopreventive activity exerted by nonsteroidal anti-inflammatory drugs (NSAIDs).23, 24 The anti-carcinogenic properties of NSAID have been attributed to their inhibition of cyclooxygenase (COX) enzymes. However, much higher doses of NSAID are needed to obtain an antitumoural effect than to inhibit COX,25 suggesting that they also act via COX-independent mechanisms. On the other hand, NSAIDs, such as aspirin, salicylate and sulindac sulphide, inhibit the proliferation of cells and induce apoptosis in various cancer cell lines, which is considered an important component of their antitumoural activity, and increased sensitization of cancer cells to anticancer drugs.26, 27, 28, 29 There is currently interest in the ability of NSAID to directly lower the levels of antiapoptotic molecules, such as the Bcl-2 family30 and 14-3-3 protein,31 which inhibits the intrinsic mitochondria-dependent apoptosis in various cancer cells. Therefore, the NSAID-induced dysfunction of antiapoptotic proteins prompted us to examine whether other antiapoptotic molecules, including Hsp70, might also be targets in the prevention of tumour progression by NSAID. In this study, we show that ibuprofen is a potent inhibitor of Hsp70, which significantly suppresses its expression by depleting heat shock factor 1 (HSF1) in lung adenocarcinoma-derived A549 cells. The downregulation of Hsp70 by ibuprofen sensitized the cells to cisplatin, which was associated with the enhancement of cisplatin-induced apoptotic signalling. Ibuprofen did not only facilitate postmitochondrial events, including the activation of cisplatin-induced caspase-9, but also the activation of Bax, causing the release of cytochrome c. Besides the demonstration of a similar increase in the sensitivity of A549 cells to cisplatin conferred by Hsp70 knockdown and ibuprofen, these observations indicate that ibuprofen accelerates cisplatin-mediated apoptosis at multiple steps of the mitochondrial apoptotic pathway via the inhibition of Hsp70. We conclude that ibuprofen is a potential chemotherapeutic agent, which might enable (a) the use of lower, less toxic does of cisplatin and (b) the design of a new combination treatment of lung cancer.
Results
Ibuprofen suppresses the expression of Hsp70 in lung adenocarcinoma cells
To define the role of Hsp70 in promoting the formation of tumours, we first examined its expression in human lung cancer cell lines. Compared with BEAS-2B, a human, non-malignant, bronchial epithelial cell line, the expression levels of Hsp70 in lung cancer cells, such as A549 and H358 adenocarcinoma, were notably higher (Figure 1a). As in previous studies, which showed an increased expression of Hsp70 in various types of human cancers, including breast, pancreas and colon, we found that Hsp70 is also dysregulated in lung cancer cells. In this study, we screened conventional NSAID in search of a new pharmacologic inhibitor, which neutralizes Hsp70, as they induce apoptosis in cancer cells by selectively downregulating antiapoptotic proteins. The expression of Hsp70 after the exposure of A549 cells to various NSAID in non-toxic concentrations, was analyzed by immunoblot. Ibuprofen, in a 400-μM concentration, decreased the expression of Hsp70 by 23% in comparison with untreated cells, whereas other NSAID had no effect (Table 1). Figure 1b shows the decrease in Hsp70 protein and mRNA levels in A549 cells, after treatment with various concentrations of ibuprofen, versus no apparent decreases in Hsc70 and Actin. Ibuprofen also decreased the expression of Hsp70 in H358, a human lung adenocarcinoma cell line, in a dose-dependent manner (Figure 1c). These results suggest that ibuprofen decreases the expression of Hsp70 in various lung cancer cell lines.
Ibuprofen enhances the apoptosis induced by cisplatin by suppressing Hsp70
As ibuprofen prominently inhibited the expression of Hsp70, we next examined its effect on the proliferation of cancer cells. We observed no significant change in the viability of A549 and H358 cells after the exposure to ∼800 μM concentrations of ibuprofen alone, which downregulates Hsp70 (Figure 2a), while the exposure to 1.0 mM concentration of ibuprofen caused cell death. Combined, these observations indicate that the downregulation of stress-inducible Hsp70 was insufficient to cause the death of A549 and H358 cells.
There is evidence that the inhibition of anti-apoptotic molecules, such as Hsp70, increases the sensitivity of tumour cells to anticancer drugs, thus improving the outcomes of chemotherapy. To study the therapeutic potential of ibuprofen, we examined whether its antitumoural effects are synergistic with those of cisplatin widely used in the treatment of lung adenocarcinoma. When we measured the survival of A549 (top of Figure 2b) and H358 (bottom of Figure 2b) cells exposed to increasing concentrations of cisplatin incubated in presence versus absence of ibuprofen, the latter prominently magnified the apoptosis induced by cisplatin, a synergistic effect confirmed by terminal deoxynucleotidyl transferase-mediated dUTP nick and labelling (TUNEL) staining (Figure 2c). To ascertain the effects conferred by the expression of Hsp70 on cell death, while excluding all effects of ibuprofen unrelated to Hsp70, we weakened the expression of Hsp70 by RNA interference (RNAi) (Figure 2d) and measured its effects on the apoptosis induced by cisplatin. The inhibition of Hsp70 decreased the viability of cisplatin-treated cells by approximately 20% (Figure 2e). Transfections with scrambled siRNA, serving as a control, showed no increase in cell death mediated by cisplatin. Cisplatin had no effect on the expression of Hsp70 (Figure 2g). We quantified the number of apoptotic cells in ibuprofen- and/or cisplatin-treated cultures using the CF488A-annexin V methods. Although cisplatin alone induced apoptosis in 10.2% of A549 cells, the co-treatment with ibuprofen increased the percentage of apoptotic cells to 34.0% (Figure 2f). These observations suggest that ibuprofen sensitizes A549 cells to cisplatin by decreasing the expression of Hsp70.
Ibuprofen decreases the expression of Hsp70 via transcriptional inactivation
The reverse transcriptase-polymerase chain reaction (RT-PCR) analysis described earlier revealed a decrease in RNA level following treatment with ibuprofen, suggesting that the expression of Hsp70 can be downregulated at the transcriptional level. After the recently discovered inhibition, by its antagonists, of the transcription of Hsp70 in cancer cells by blockade of the activation of HSF118, 20 (which is often upregulated and constitutively activated in tumour formation), we studied the effects of ibuprofen on HSF1 in A549 cells. We first performed a ChIP assay to explore whether the inhibitory effect of ibuprofen is at the level of HSF1 DNA binding. As expected, we found an unequivocal association between HSF1 and the Hsp70 gene promoter containing the HSE site, in ibuprofen-untreated cells (Figure 3a). It is noteworthy that ibuprofen eliminated this binding (Figure 3a), suggesting that it inhibits the expression of Hsp70 via the action of HSF1. This also suggests that ibuprofen blocks the binding of HSF1 chromatin, or the steps which precede, in several processes needed to activate HSF1. Therefore, we broadened our analysis to examine the effect of ibuprofen on the expression of HSF1. Compared with unexposed, control cells, the HSF1 mRNA level was significantly lower in cells exposed to ibuprofen (bottom of Figure 3b). Consistent with its effect on the expression of mRNA, ibuprofen also decreased the expression of HSF1 protein in a dose-dependent fashion (top of Figure 3b). To confirm the inhibition of HSF1-mediated Hsp70 by ibuprofen, we lowered the amounts of HSF1 present in A549 cells by RNAi, and studied its effect on the expression of Hsp70. The treatment of cells with HSF1 dsRNA decreased the Hsp70 level compared with that measured in cells untreated with dsRNA (Figure 3c). Ibuprofen decreased the expression of HSF1 by 16% in comparison with untreated cells, whereas other NSAID had no effect (Table 2). Overall, these observations indicate that ibuprofen inhibited the expression of Hsp70 by depleting the HSF1 in A549 cells.
Ibuprofen accelerates the mitochondrial apoptotic process induced by cisplatin
Several studies have found that mitochondria might be a direct and important target of cisplatin in sensitive cells.32, 33 We studied the effects of ibuprofen on the depolarization of mitochondrial membranes and the cytochrome c release induced by cisplatin. A549 cells with or without cisplatin were incubated in absence or presence of ibuprofen and stained with JC-1. Treatment with cisplatin and ibuprofen lowered the mitochondrial membrane potential, manifest by an attenuated red and an enhanced green mitochondrial fluorescence (Figure 4a, lower right panel) compared with that observed with cisplatin alone (Figure 4a, upper right panel), while control (Figure 4a, upper left panel) or ibuprofen alone (Figure 4a, lower left panel) produced the red-dotted staining pattern of polarized mitochondria. The intensity of green mitochondrial fluorescence in cisplatin-treated cells is significantly increased (36.56 to 55.56%) by the co-treatment with ibuprofen. Ibuprofen also promoted the release of cytochrome c from the mitochondria induced by cisplatin (Figure 4b). These findings unequivocally indicated that, in A549 cells, ibuprofen enhanced the mitochondria-dependent apoptosis caused by cisplatin.
Ibuprofen increases the activation of Bax induced by cisplatin
The translocation of the pro-apoptotic protein Bax to the mitochondria is closely associated with the apoptosis induced by cisplatin. To explore the mechanisms by which ibuprofen promotes the apoptosis mediated by mitochondria in response to cisplatin, we examined whether it was due to its ability to stimulate the translocation of Bax by cisplatin. We first monitored conformational changes in Bax as indicators of its activation. Western blot analysis of the immunoprecipitates with a conformation specific anti-Bax (6A7) antibody, which only recognizes the active form, revealed the presence of active Bax in A549 cells treated with cisplatin (Figure 5a, lane 4), although not in untreated cells (Figure 5a, lanes 1 and 2). Further exposure of the cisplatin-treated cells to ibuprofen caused a 1.5-fold increase in active Bax, compared with incubation with cisplatin alone (Figure 5a, lane 3). When we analyzed the effects conferred by ibuprofen on the translocation of Bax to mitochondria in cisplatin-treated cells, we observed an approximately 1.3-fold increase in the amount of translocated Bax (Figure 5b). To exclude an effect of ibuprofen unrelated to the inhibition of Hsp70, we performed RNAi for a selective knock-down of Hsp70, and we studied its effects on the activation of Bax. Consistent with the earlier data presented for ibuprofen, the depletion of Hsp70 increased the activation of Bax in cisplatin-treated cells, although its extent was greater with Hsp70 RNAi than with ibuprofen (Figure 5c). These observations confirmed that (a) ibuprofen promotes the activation of Bax dependent on cisplatin and its translocation to the mitochondria in A549 cells and (b) its mechanism of action is mediated by the inhibition of Hsp70.
Ibuprofen facilitates events occurring upstream and downstream of mitochondrial disruption in cisplatin-mediated apoptosis
Previous studies have shown that Hsp70 can inhibit apoptosis by acting downstream of the mitochondria.12, 13, 14, 15 Hsp70 interacts directly with Apaf-1 to prevent the formation of cytochrome c-mediated apoptosome and subsequent activation of caspase-9. To examine whether ibuprofen also influences the downstream mitochondrial events, we measured its effects on the cleavage of procaspase-9 in the apoptosis mediated by cisplatin. With an anti-active caspase-9 antibody, fully processed caspase-9 was predominantly identified in cisplatin-treated A549 cells (Figure 6a, lane 3) over untreated cells (Figure 6a, lanes 1 and 2). It is noteworthy that treatment with ibuprofen increased >4-fold the amount of active caspase-9 in cells treated with cisplatin, compared with cells incubated with cisplatin alone (Figure 6a, lane 4). As, as reported earlier, the highest increases in the activation of Bax and release of cytochrome c by ibuprofen were <2-fold, these observations suggest that ibuprofen also facilitates the post mitochondrial process taking place between the release of cytochrome c and the activation of caspase-9. To verify that this is a specific effect, we studied the effect of Hsp70 knock-down on the activation of caspase-9 mediated by cisplatin. The caspase-9 activity in cells depleted of Hsp70 with cisplatin was fourfold greater than in control (scrambled) siRNA-treated cells (Figure 6b). We obtained similar results when we measured the activity of caspase-9 in cells treated with ibuprofen (Figure 6c) or siRNA against Hsp70 (Figure 6d) by a fluorometric assay using a synthetic substrate. Overall, these observations confirmed unambiguously that ibuprofen intensified the apoptosis induced by cisplatin by its effects on the events occurring downstream of the mitochondria, by inhibiting Hsp70, although whether it stimulated the formation of apoptosome (essential for the recruitment of procaspase-9) remains to be determined. We conclude that ibuprofen promotes the apoptosis induced by cisplatin at multiple stages of the mitochondrial cascade, by attenuating the expression of Hsp70 in A549 cells.
Discussion
We found that, compared with non-malignant bronchial epithelial cells, human lung cancer cells overexpressed Hsp70. This is an important observation, as targeting the expression or function of Hsp70 has been suggested as an effective treatment strategy in several cancers, based on the hypothesis that higher levels of Hsp70 protect against cell death and increase the survival rate against modalities used in chemotherapy.11, 15 In fact, it is well documented that the expression of Hsp70 is significantly increased in cancer tissues and/or serums obtained from patients with non-small cell lung cancer (NSCLC)34, 35, 36, 37, 38 and its overexpression correlates with poor prognosis in NSCLC.36 Several reports have indicated that functionally related small molecules that inhibit Hsp70 decrease the viability of colo-rectal or pancreatic cancer cells by promoting apoptosis via the downregulation of Hsp70, and may be a promising new class of cancer chemotherapeutics.19, 21, 22 We showed that ibuprofen, a relatively non-toxic and widely used NSAID, significantly decreased the expression of Hsp70 in lung adenocarcinoma cell lines. We also clearly demonstrated that the inhibitory mechanisms of ibuprofen on Hsp70 are due to a decrease in HSF1 expression. Although the fundamental mechanism behind the reduction in HSF-1 expression is unknown, a previous study has indicated that the nuclear factor 1 family member NFIX, which codes for site-specific DNA-binding proteins known to have multiple roles in replication, signal transduction and transcription, exerts a transcriptional repressive effect on the expression of HSF1 in cancer cells.39 Whether NFIX is, indeed, involved in the inhibition of HSF1 expression evoked by ibuprofen is applicable in further studies. To the best of our knowledge, this is the first study of the inhibitory effects of NSAID on the cellular expression of Hsp70. In addition, we showed that ibuprofen does not influence the cell viability without additional stimuli, unlike its maximal effect on the expression of Hsp70. The lack of inhibitory efficacy of ibuprofen against tumours is consistent with a previous study, which showed that low-dose ibuprofen did not induce apoptosis in mouse and human colorectal cancer cell lines.29 Similar observations were made following RNAi of Hsp70, suggesting that the attenuation of Hsp70 per se is insufficient to cause the death of A549, and perhaps other cells. It has been shown that the knockdown of Hsp70 has no effect on the viability of several cancer cell lines, although sensitized them to anticancer drugs.40, 41 Therefore, the therapeutic potential of ibuprofen combined with chemotherapeutic agents needs to be explored.
Cisplatin is one of most effective chemotherapeutic drugs against NSCLCs.42 It is noteworthy that damage to DNA caused by cisplatin enables apoptosis involving mitochondrial pathways, which is negatively regulated by Hsp70. As ibuprofen prominently suppressed the expression of Hsp70 in A549 and H358 cells, we examined the possible synergistic activity of ibuprofen and cisplatin against cancer. As expected, ibuprofen potentiated synergistically the anti-proliferative effect of cisplatin in A549 and H358 cells. Despite its potent antitumoural properties, the therapeutic use of cisplatin in oncology is seriously limited by dose-dependent adverse effects and frequent development of drug resistance.43 Therefore, our findings may make useful contributions toward the development of new and less toxic chemotherapy against NSCLCs.
We also examined the molecular mechanisms of these synergistic properties of ibuprofen. Hsp70 protects cells against mitochondria-dependent apoptosis at different levels, although the precise mechanism remains hypothetical, because of regular contradictory descriptions of Hsp70 function. Earlier reports have shown a protective effect of Hsp70 against cellular apoptosis by inhibition of the apoptosome function, a protein complex comprising Apaf-1 and cytochrome c.12, 13 However, recent reports have questioned this repression of apoptosis downstream of the mitochondrial membrane permeabilization. Several studies have suggested that Hsp70 functions upstream of the mitochondria by preventing the release of cytochrome c, instead of inhibiting the apoptosome or other downstream points in the caspase cascade.16, 17 Some of this confusion may be due to different experimental systems used to evaluate apoptosis, or reflects the variability of apoptotic pathways among different cell lines. In this study, we found that the inhibition of Hsp70 by ibuprofen facilitates the activation of Bax induced by cisplatin, and its translocation to the mitochondria in A549 cells. This finding is consistent with the previous observation of blockade of Bax activation being one of the upstream sites of action of Hsp70. On the other hand, the role played by Hsp70 in the A549 cellular mitochondrial apoptotic pathway is likely to be more complex than described earlier because, in cisplatin-treated cells, the decreased expression of Hsp70 caused by ibuprofen amplified the activation of caspase-9 significantly compared with that of Bax. Furthermore, the similar increase in the activation of cisplatin-dependent Bax and release of cytochrome c by ibuprofen suggests that Hsp70 also inhibits the post mitochondrial steps between the release of cytochrome c and the activation of caspase-9. If Hsp70 were acting downstream of the mitochondria, one would predict that it interferes with the activation of caspase-9 in response to cytochrome c, either by inhibiting the formation of the apoptosome, or by preventing the binding of pro-caspase-9 to this complex. When we studied the effects of Hsp70 on the formation of, and recruitment of pro-caspase-9 to, the apoptosome, the cell lysates were immunoprecipitated, although Hsp70 failed to migrate with Apaf-1, cytochrome c or caspase-9 (data not shown). These results may be supported by previous report that no association between Hsp70 and Apaf-1 or apoptosome complex was observed, even under in vitro activation of caspase by the addition of cytochrome c and dATP.44 Furthermore, we were unable to identify a new target for Hsp70 in the process of caspase-9 activation, indicating that its inhibitory activity is attributable to another indirect effect, instead of a direct one as previously reported. Altogether, the data presented here are the first evidence of cell death inhibition by Hsp70, by its targeting of both upstream and downstream mitochondrial processes, while the precise mechanisms by which it interferes with the activation of caspase-9 remains to be clarified.
In conclusion, ibuprofen potentiated the antitumoural properties of cisplatin in the cells of lung adenocarcinoma, via a mechanism of action mediated by the suppression of Hsp70. These findings may promote the development of a new strategy to increase the effectiveness of cisplatin in the treatment of NSCLCs, as well as highlight the putative merits of developing anticancer treatments targeting Hsp70.
Materials and Methods
Materials
The mouse monoclonal anti-Hsp70, the rat monoclonal anti-Hsc70 and rabbit polyclonal HSF-1 antibodies purchased from Stressgen – Enzo Life Sciences, Inc., Plymouth Meeting, PA, USA. Anti-Bax rabbit polyclonal (N-20) and anti-VDAC-1 goat polyclonal (N-18) antibodies were purchased from Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA. Anti-cytochrome c mouse monoclonal antibody (556433) was obtained from BD Pharmingen Inc., San Diego, CA, USA. Anti-caspase 9 and -ERK antibodies were acquired from Cell Signaling Technology Inc., Danvers, MA, USA. The mouse monoclonal antibody against actin was obtained from Chemicon International Inc., Temecula, CA, USA. Anti-Bax 6A7 monoclonal antibody and other reagents were purchased from Sigma-Aldrich, St. Louis, MO, USA.
Cell culture and viability assay
A549 and H358 lung cancer cell lines were cultured in Dulbecco’s modified Eagle’s medium containing 10% foetal bovine serum at 37 °C. BEAS-2B cells were grown in bronchial epithelial basal medium. All NSAID and cisplatin were dissolved in dimethyl sulphoxide and added to the medium at indicated concentrations. The activity of mitochondrial dehydrogenase 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure cell death/survival. The reaction product was measured at A570, and the relative viability of cells treated with reagents versus untreated cells was calculated.
TUNEL staining
The TUNEL assay was performed using an in situ cell death detection kit (F Hoffmann-La Roche, Basel, Switzerland) according to the manufacturer’s instructions. The ratio of TUNEL-positive cells to the total number of cells was calculated.
Immunoprecipitation and cell fractionation
A549 cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM sodium orthovanadate, 1 mM EDTA, 0.1% NP-40, 10 mM NaF) containing the Calbiochem Protease Inhibitor Cocktail Set III (Merck KGaA, Darmstadt, Germany). The cell lysates and immunoprecipitates were resolved in Laemmli sample buffer. The samples underwent sodium dodecyl sulphate-polyacrylamide gel electrophoresis were transferred to a polyvinylidene difluoride membrane, reacted with the respective antibodies, and detected with an ECL chemiluminescence detection kit (GE Healthcare, Fairfield, CT, USA). For the immunoprecipitation, the cell lysates were incubated with the indicated antibodies for 1 h at 4 °C. Protein G-sepharose beads were added to collect the immunocomplexes for an additional 1 h of incubation. The pellets were washed three times with lysis buffer. The mitochondria and cytosol fractions were prepared as described previously.45
Chromatin immunoprecipitation (ChIP) assay
ChIP assays were performed as described previously,46 using an EZ ChIP kit (Upstate Biotechnology Inc., Waltham, MA, USA). Briefly, after adding formaldehyde, A549 cells were suspended in SDS lysis buffer and the chromatin DNA was disrupted by sonication. For the immunoprecipitation, the lysate was incubated with anti-HSF-1 antibody, followed by immobilization on salmon sperm DNA/Protein G agarose. The protein/DNA complexes extracted with elution buffer were heated to 65 °C for 6 h to reverse cross-links, then digested with proteinase K. DNA fragments were amplified in PCR with the ChIP assay primers containing the heat shock element sites in human Hsp70 promoter. PCR primers for the ChIP assay were as follows: Hsp70 (−103/+7) (F) 5′-TGATTGGTCCAAGGAAGGCT-3′ and (R) 5′-AAAAAGGTAGTGGACTGTCGC-3′.
Reverse transcriptase-PCR
RT-PCR was carried out, using a Qiagen (Valencia, CA, USA) One-Step RT-PCR Kit. We used the following primer pairs to amplify. Hsp70, (F) 5′-ATGAAGCACTGGCCTTTCCA-3′, (R) 5′-TTGTTCTGGCTGATGTCCTT-3′; Hsc70, (F) 5′-TGGAACTATTGCTGGTCTCAA3′; (R) 5′-AGAACCACCAACCAGGACAAT-3′; HSF-1, (F) 5′-TTCGACCAGGGCCAGTTT-3′; (R) 5′-AGAGCTGGCCACAGCATCA-3′; actin, (F) 5′-AGAGGCATCCTCACCCTGA-3′; (R) 5′-CATCTCTTGCTCGAAGTCCA-3′. The products were examined by agarose gel electrophoresis after 23 cycles.
RNA interference
The sequences of the sense strands used to generate specific siRNA were obtained as follows: HSF-1, 5′-AAGTACTTCAAGCACAACAA-3′, 5′-AAGAGTGAAGACATAAAGAT-3′, 5′-AAGTCGTCAACAAGCTCATT-3′. The siRNAs were synthesized using the Silencer siRNA construction kit (Ambion; Applied Biosystems Inc., Carlsbad, CA, USA). Double-stranded Hsp70 and control siRNA duplex were synthesized as followed by Qiagen: Hsp70-specific sequence, 5′-CCAUUGAGGAGGUAGAUUAdTdT-3′. A549 cells were transfected with each siRNA (10 nmol/l) using the Lipofectamine 2000 (Invitrogen; Applied Biosystems Inc.), and grown for 72 h to allow an effective decrease in the expression of the respective target molecules.
Quantification of apoptosis by flow cytometry
A549 cells were washed with Annexin V staining buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1.8 mM CaCl2), and incubated with CF488A-Annexin V and propidium iodide (Biotium, Inc., Hayward, CA, USA) in staining buffer for 30 min at 37 °C in the dark. Fluorescence was measured using a FACSCalibur (BD Biosciences, San Jose, CA, USA), and the data were analyzed with CellQuest software (BD Biosciences).
JC-1 staining and quantification
A549 cells were cultured at 37 °C for 48 h on glass chamber slides and treated with ibuprofen and cisplatin at the specified concentrations for 48 h. Mitochondrial permeability transition was determined by staining the cells with 5,5′,6,6′-tetrachloro-1,1,3,3′-tetraethyl- benzimidazolylcarbocyanin iodide (JC-1; Molecular Probes, Invitrogen, Carlsbad, CA, USA) in the dark. The cells were subsequently washed with assay buffer according to the manufacturer’s protocol and immediately imaged using a fluorescence microscope (Keyence Corporation, Osaka, Japan) with the red (λexcitation: 560±40 nm band pass filter, λdetection: 630±60 nm band pass filter) and green (λexcitation: 470±40 nm band pass filter, λdetection: 535±50 nm band pass filter) fluorescence channels. Flow cytometric analysis was assayed with the JC-1 Mitochondrial Membrane Potential Kit (AAT Bioquest, Sunnyvale, CA, USA) according to the manufacturer’s directions, using a FACSCalibur, and the results were analyzed by CellQuest software.
In vitro casapse-9 activity determination
Caspase-9 activity was measured by a fluorometric assay in whole-cell lysates using Ac-Leu-Glu-His-Asp-MCA substrate (Peptide International Inc., Louisville, KY, USA). A549 cell extracts were mixed with Ac-LEHD-MCA in ICE standard buffer (100 mM HEPES pH 7.5, 10% sucrose, 0.1% CHAPS, 10 mM DTT, 1 mM PMSF), and cleavage of the fluorogenic peptide substrate was monitored at 37 °C for 30 min by a SPECTRA max GEMINI EM (Molecular Device, Sunnyvale, CA, USA) fluorometer with excitation at 370 nm and emission at 460 nm.
Abbreviations
- Apaf-1:
-
apoptotic protease-activating factor 1
- COX:
-
cyclooxygenase
- HSF-1:
-
heat shock factor 1
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- NSAID:
-
nonsteroidal anti-inflammatory drug
- NSCLC:
-
non-small cell lung cancer
- PCR:
-
polymerase chain reaction
- RNAi:
-
RNA interference
- RT:
-
reverse transcriptase
- TUNEL:
-
terminal deoxynucleotidyl transferase-mediated dUTP nick and labelling
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This work was supported, in part, by a Grant-in-Aid (23591477) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Endo, H., Yano, M., Okumura, Y. et al. Ibuprofen enhances the anticancer activity of cisplatin in lung cancer cells by inhibiting the heat shock protein 70. Cell Death Dis 5, e1027 (2014). https://doi.org/10.1038/cddis.2013.550
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DOI: https://doi.org/10.1038/cddis.2013.550
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