Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Product Review
  • Published:

High-resolution mass spectrometer for protein chemistry

Nature volume 370, pages 393–395 (1994)Cite this article

Abstract

Matrix-assisted laser desorption/ionization is an accurate and sensitive method for measuring the molecular weights of peptides and proteins. Usually time-of-flight mass spectrometry is used to detect the laser-produced ions, but a new method called Fourier transform mass spectrometry offers greater sensitivity and much higher mass measurement accuracy.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Karas, M., Bachmann, D., Bahr, U. & Hillenkamp, F. Int. J. Mass Spectrom. Ion Processes 78, 53–68 (1987).

    Article  ADS  CAS  Google Scholar 

  2. Karas, M. & Hillenkamp, F. Analyt. Chem. 60, 2291–2301 (1988).

    Article  Google Scholar 

  3. Karas, M., Bahr, U. & Hillenkamp, F. Int. J. Mass Spectrom. Ion Processes 92, 231–242 (1989).

    Article  ADS  CAS  Google Scholar 

  4. Hillenkamp, F., Karas, M., Beavis, R. C. & Chait, B. T. Analyt. Chem. 63, 1193A–1203A (1991).

    Article  CAS  Google Scholar 

  5. Billeci, T. M. & Stults, J. T. Analyt. Chem. 65, 1709–1716 (1993).

    Article  CAS  Google Scholar 

  6. Chait, B. T. & Kent, S. B. H. Science 257, 1885–1894 (1992).

    Article  ADS  CAS  Google Scholar 

  7. Biemann, K. A. Rev. Biochem. 30, 977–1010 (1992).

    Article  Google Scholar 

  8. Chait, B. T., Wong, R., Beavis, R. & Kent, S. B. H. Science 262, 89–92 (1993).

    Article  ADS  CAS  Google Scholar 

  9. Henzel, W. J., Billeci, T. M., Stults, J. T., Wong, S. C., Grimley, G. & Watanabe, C. Proc. natn. Acad. Sci. U.S.A. 90, 5011–5015 (1993).

    Article  ADS  CAS  Google Scholar 

  10. Mann, M., Højrup, P. & Roepstorff, P. Bio. Mass Spectrom. 22, 338–245 (1993).

    Article  CAS  Google Scholar 

  11. Arnott, D., Shabanowitz, J. & Hunt, D. Clin. Chem. 39, 2005–2010 (1993).

    CAS  PubMed  Google Scholar 

  12. Cotter, R. C. Analyt. Chem. 64, 1027A–1039A (1992).

    Article  CAS  Google Scholar 

  13. Beavis, R. C. & Chait, B. T. Chem. Phys. Lett. 181, 479–484 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Zhou, J., Ens, W., Standing, K. C. & Verentchikov, A. Rapid Commun. Mass Spectrom. 6, 671–678 (1992).

    Article  ADS  CAS  Google Scholar 

  15. Pan, Y. & Cotter, R. J. Org. Mass Spectrom. 27, 3–8 (1992).

    Article  CAS  Google Scholar 

  16. Spengler, B., Kirsch, D. & Kaufmann, R. Rapid Commun. Mass Spectrom. 5, 198–202 (1991).

    Article  ADS  CAS  Google Scholar 

  17. Mclver, R. T., Jr, Li, Y. & Hunter, R. L. Proc. natn. Acad. Sci. U.S.A. 91, 4801–4805 (1994).

    Article  ADS  Google Scholar 

  18. Mclver, R. T., Jr, Li, Y. & Hunter, R. L. Rapid Commun. Mass Spectrom. 8, 237–241 (1994).

    Article  ADS  Google Scholar 

  19. Mclver, R. T., Jr, Li, Y. & Hunter, R. L. Int. J. Mass Spectrom. Ion Processes 132, L1–L7 (1994).

    Article  ADS  Google Scholar 

  20. Li, Y., Mclver, R. T., Jr & Hunter, R. L. Analyt. Chem. 66, 2077–2083 (1994).

    Article  CAS  Google Scholar 

  21. Comisarow, M. B. & Marshall, A. G. Chem. Phys. Lett. 26, 489–490 (1974).

    Article  ADS  CAS  Google Scholar 

  22. Marshall, A. G. Accts chem. Res. 18, 316–322 (1985).

    Article  CAS  Google Scholar 

  23. Comisarow, M. B. J. chem. Phys. 69, 4097–4104 (1978).

    Article  ADS  CAS  Google Scholar 

  24. Mclver, R. T., Jr Hunter, R. L., Ledford, E. B., Locke, M. J. & Francl, T. J. Int. J. Mass Spectrom. Ion Processes 39, 65–84 (1981).

    Article  ADS  Google Scholar 

  25. Wineland, D., Ekstrom, P. & Dehmelt, H. Phys. Rev. Lett. 31, 1279–1282 (1973).

    Article  ADS  CAS  Google Scholar 

  26. Ekstrom, P. ; Wineland, D. Scient. Am. 243, 105–119 (1980).

    Article  CAS  Google Scholar 

  27. Strupat, K., Karas, M. & Hillenkamp, F. Int. J. Mass Spectrom. Ion Processes 111, 89–102 (1991).

    Article  ADS  CAS  Google Scholar 

  28. Mclver, R. T., Jr . US Patent 4,535,235 issued 13 August, 1985.

  29. Mclver, R. T., Jr . Hunter, R. L. & Bowers, W. D. Int. J. Mass Spectrom. Ion Processes 64, 67–77 (1985).

    Article  ADS  Google Scholar 

  30. Lebrilla, C. B., Amster, I. J. & Mclver, R. T., Jr Int. J. Mass Spectrom. Ion Processes 87, R7–R13 (1989).

    Article  ADS  CAS  Google Scholar 

  31. Lebrilla, C. B., Wang, D. T.-S., Mizoguchi, T. J. & Mclver, R. T., Jr J. Am. chem. Soc. 111, 8593–8598 (1989).

    Article  CAS  Google Scholar 

  32. Mclver, R. T., Jr Int. J. Mass Spectrom. Ion Processes 98, 35–50 (1990).

    Article  ADS  Google Scholar 

  33. Beavis, R. C. & Chait, B. T. Rapid Commun. Mass Spectrom. 3, 432–435 (1989).

    Article  ADS  CAS  Google Scholar 

  34. Schwartz, J. C. & Bier, M. E. Rapid Commun. Mass Spectrom. 7, 27–32 (1993).

    Article  ADS  CAS  Google Scholar 

  35. Chambers, D. M., Goeringer, D. E., McLuckey, S. A. & Glish, G. L. Analyt. Chem. 65, 14–20 (1993).

    Article  CAS  Google Scholar 

Download references

Authors
  1. Yunzhi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard L. Hunter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert T. Mclver
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, Y., Hunter, R. & Mclver, R. High-resolution mass spectrometer for protein chemistry. Nature 370, 393–395 (1994). https://doi.org/10.1038/370393a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/370393a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing