>A system is described for accurately translating the positive signal, obtained after hybridization of high-density colony filters, into clone coordinates. Calibration marks are made on the filter at the same time and in register with the clones. A combination of digitizing tablet and software allows for the retrieval of clone coordinates directly from the autoradiograph.
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Jones, C. Systematic identification of clone coordinates from high-density filters. Nature 365, 369–370 (1993). https://doi.org/10.1038/365369a0
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DOI: https://doi.org/10.1038/365369a0