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SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast

Nature volume 357, pages 505–508 (1992)Cite this article

Abstract

IN budding yeast many genes are expressed under cell-cycle control in late Gl. These include a large group of DNA synthesis genes1, the HO gene2 involved in mating-type switching, CTS1 (chitinase)3 and also CLN1 and CLN2 (ref. 4) encoding Gl cyclins. Two factors, encoded by the SWI4 and SWI6genes, are required for HO (ref. 5), CLN (refs 6, 7) andCTS1 (ref. 3) gene expression and, at least in the HO promoter, bind to CACGA4 upstream sequences5,7–9(CCBs). This motif is not found upstream of the DNA synthesis genes, which instead have a hexamer element, ACGCGT1 (MCB), an MluI restriction site, that is recognized by a cell-cycle regulated transcription complex DSC1 (ref. 1). This Mlu I-activation system consisting of the MCBs and DSC1 is conserved in fission yeast where a DSCl-like complex controls the cdc22+ribonucleotide reductase gene10. The Schizosae-charomyces pombe cdc10+ gene encodes a component of DSC1 (ref. 10) and, significantly, this has homology with both the Swi4and Swi6 proteins 8,11. Here we show that Swi6 is an essential component of DSC1 and that deletion of SWI6 impairs the cell-cycle regulation of the DNA synthesis genes, as well as CLN1 and CLN2. Thus Swi6 is the common factor in regulation of all the above genes and may therefore be responsible for the timing of their expression in late Gl.

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References

  1. Lowndes, N. F., Johnson, A. L. & Johnston, L. H. Nature 350, 247–250 (1991).

    Article  ADS  CAS  Google Scholar 

  2. Nasmyth, K. A. Nature 302, 670–676 (1983).

    Article  ADS  CAS  Google Scholar 

  3. Dohrmann, P. R. et al. Genes Dev. (in the press).

  4. Wittenberg, C., Sugimoto, K. & Reed, S. I. Cell 62, 225–237 (1990).

    Article  CAS  Google Scholar 

  5. Breeden, L. & Nasmyth, K. A. Cell 48, 389–397 (1987).

    Article  CAS  Google Scholar 

  6. Nasmyth, K. A. & Dirick, L. Cell 66, 995–1013 (1991).

    Article  CAS  Google Scholar 

  7. Ogas, J., Andrews, B. J. & Herskowitz, I. Cell 66, 1015–1026 (1991).

    Article  CAS  Google Scholar 

  8. Andrews, B. J. & Herskowitz, I. Nature 342, 830–833 (1989).

    Article  ADS  CAS  Google Scholar 

  9. Taba, M. R. M., Muroff, I., Lydall, D., Tebb, G. & Nasmyth, K. A. Genes Dev. 5, 2000–2013 (1991).

    Article  CAS  Google Scholar 

  10. Lowndes, N. F., Mclnerny, C. J., Johnson, A. L., Fantes, P. A. & Johnston, L. H. Nature 355, 449–453 (1992).

    Article  ADS  CAS  Google Scholar 

  11. Breeden, L. & Nasmyth, K. A. Nature 329, 651–654 (1987).

    Article  ADS  CAS  Google Scholar 

  12. Mclntosh, E., Atkinson, T., Storms, R. K. & Smith, M. Molec. cell. Biol. 11, 329–337 (1991).

    Article  Google Scholar 

  13. Johnston, L. H., Lowndes, N. F., Johnson, A. L. & Sugino, A. Cold Spring Harb. Symp. quant. Biol. (in the press).

  14. Breeden, L. & Mikesell, G. E. Genes Dev. 5, 1183–1190 (1991).

    Article  CAS  Google Scholar 

  15. Simanis, V. & Nurse, P. J. Cell Sci. 92, 51–56 (1989).

    CAS  PubMed  Google Scholar 

  16. Nasmyth, K. A., Seddon, A. & Ammerer, G. Cell 49, 549–558 (1987).

    Article  CAS  Google Scholar 

  17. Surana, U. et al. Cell 65, 145–161 (1991).

    Article  CAS  Google Scholar 

  18. Ghiara, J. B. et al. Cell 65, 163–174 (1991).

    Article  CAS  Google Scholar 

  19. Johnston, L. H., Eberly, S. L., Chapman, J. W., Araki, H. & Sugino, A. Molec. cell. Biol. 10, 1358–1366 (1990).

    Article  CAS  Google Scholar 

  20. Guarente, L. & Mason, T. Cell 32, 1279–1286 (1983).

    Article  CAS  Google Scholar 

  21. Barker, D. G., White, J. H. M. & Johnston, L. H. Nucleic Acids Res. 13, 8323–8336 (1985).

    Article  CAS  Google Scholar 

  22. White, J. H. M., Barker, D. G., Nurse, P. & Johnston, L. H. EMBO J. 5, 1705–1709 (1986).

    Article  CAS  Google Scholar 

  23. Lowndes, N. F. & Johnston, L. H. Trends Genet. 8, 79–81 (1992).

    Article  CAS  Google Scholar 

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  1. Leland H. Johnston: To whom correspondence should be addressed.

Authors and Affiliations

  1. Laboratory of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

    Noel F. Lowndes, Anthony L. Johnson & Leland H. Johnston

  2. Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104, USA

    Linda Breeden

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  1. Noel F. Lowndes
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  2. Anthony L. Johnson
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  3. Linda Breeden
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  4. Leland H. Johnston
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Lowndes, N., Johnson, A., Breeden, L. et al. SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast. Nature 357, 505–508 (1992). https://doi.org/10.1038/357505a0

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