Abstract
THE α1 and α2 domains of major histocompatibility complex (MHC) class I molecules function in the binding and presentation of foreign peptides to the T-cell antigen receptor and control both negative and positive selection of the T-cell repertoire1–3. Although the α3 domain of class I is not involved in peptide binding, it does interact with the T-cell accessory molecule, CDS (refs 4, 5). CDS is important in the selection of T cells as anti-CDS antibody injected into perinatal mice interfers with this process6. We previously used a hybrid class I molecule with the α1/α2 domains from Ld and the α3 domain from Q7b and showed that this molecule binds an Ld-restricted peptide but does not interact with CD8-dependent cytotoxic T lymphocytes7. Expression of this molecule in transgenic mice fails to negatively select a subpopulation of anti-Ld cytotoxic T lymphocytes. In addition, positive selection of virus-specific Ld-restricted cytotoxic T lymphocytes does not occur. We conclude that besides the α1/α2 domains of class I, the α3 domain plays an important part in both positive and negative selection of antigen-specific cells.
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References
Bjorkman, P. J. et al. Nature 329, 506–518 (1987).
Marusic-Galesic, S., Stephany, D. A., Longo, D. L. & Kruisbeek, A. M. Nature 333, 180–183 (1988).
Teh, H. S. et al. Nature 335, 229–233, (1988).
Connolly, J. M., Potter, T. A., Wormstall, E. & Hansen, T. H. J. exp. Med. 168, 325–341 (1988).
Salter, R. et al. Nature 345, 41–46 (1990).
Zuniga-Pflucker, J. C., Jones, L. A., Longo, D. L. & Kruisbeek, A. M. J. exp. Med. 171, 427–437 (1990).
Aldrich, C. J. et al. J. Immun. 146, 3082–3090 (1991).
Stroynowski, I. A. Rev. Immun. 8, 501–530 (1990).
Sprent, J. & Schaeffer, M. Immun. Rev. 117, 213–234 (1990).
Alexander, M. A., Damico, C. A., Wieties, K. M., Hansen, T. H. & Connolly, J. M. J. exp. Med. 173, 849–858 (1991).
MacDonald, H. R., Glasebrook, A. L., Bron, C., Kelso, A. & Cerottini, J.-C. Immun. Rev. 68, 89–115 (1982).
Budd, R. C., Cerottini, J. C. & MacDonald, J. I. J. Immun. 138, 1009–1013 (1987).
Reddehase, M., Rothbard, J. B. & Koszinowski, U. H. Nature 337, 651–653 (1989).
Lie, W. R. et al. Nature 344, 439–441 (1990).
Lie, W. R. et al. J. exp. Med. 173, 449–459 (1991).
Norment, A. M., Salter, R. D., Parham, P., Engelhard, V. H. & Littman, D. R. Nature 336, 79–81 (1988).
Zamoyska, R. R. et al. Nature 342, 278–281 (1989).
Teh, H. S., Kishi, H., Scott, B. & von Boehmer, H. J. exp. Med. 169, 795–806 (1989).
Fung-Leung, W.-P. et al. Cell. 65, 443–449 (1991).
Teh, H. S. et al. Nature 349, 241–243 (1990).
Qin, S., Cobbold, S., Benjamin, R. & Waldmann, H. J. exp. Med. 169, 779–794 (1989).
Aldrich, C. J., Jenkins, R. N. & Rich, R. R. J. Immun. 136, 383–388 (1986).
Trowbridge, I. S., Lesley, J., Schulte, R., Hyman, R. & Trotter, J. Immunogenetics 15, 299–306 (1982).
Koszinowski, U. H., Keil, G. M., Schwarz, H., Schickedanz, J. & Reddehase, M. J. J. exp. Med. 166, 289–294 (1987).
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Aldrich, C., Hammer, R., Jones-Youngblood, S. et al. Negative and positive selection of antigen-specific cytotoxic T lymphocytes affected by the α3 domain of MHC I molecules. Nature 352, 718–721 (1991). https://doi.org/10.1038/352718a0
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DOI: https://doi.org/10.1038/352718a0
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