The use of short microbore HPLC columns with different selectivity for the purification of proteins and peptides at the picomole level is discussed.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Esch, F. S. Analyt. Biochem. 136, 39–47 (1984).
Hunkapiller, M.W. & Hood, L.E. Science 219, 650–659 (1983).
Kucera, P. J. Chromatogr. 198, 93 (1980).
Nice, E.C., Lloyd, C.J. & Burgess, A.W. J. Chromatogr. 296, 153–170 (1984).
Moore, R.M. & Walters, R.R. J. Chromatogr. 317, 119–128 (1984).
Nice, E.C., Grego, B. & Simpson, R. Biochem. Int. 11, 187–195 (1985).
Simpson, R.J., Moritz, R.L., Begg, G.S., Rubira, M.R. & Nice, E.C. Analyt. Biochem. 177, 221–236 (1989).
Simpson, R.J., Moritz, R.L., Nice, E.C. & Grego, B. Eur. J. Biochem. 165, 21–29 (1987).
Simpson, R.J. & Moritz, R.L. J. Chromatogr. 474, 418–423 (1989).
Rubenstein, M. Analyt. Biochem. 98, 1–7 (1979).
Bij, K.E., Horváth, Cs., Melander, W.R. & Naham, A. J. Chromatogr. 203, 65–84 (1985).
Hearn, M.T.W. & Grego, B. J. Chromatogr. 255, 125–136 (1981).
Ward, L.D., Reid, G.E., Moritz, R.L. & Simpson, R.J. in Current Research in Protein Chemistry (ed. Villafrance, J.J.) (Academic, New York, 1990).
Nice, E.C. & Simpson, R.J. J. Pharmaceut. Biomed. Anal. 7, 1039–1053 (1989).
Simpson, R.J. & Nice, E.C. in The Use of HPLC in Receptor Biochemistry (ed. Kerlavage, A.R.) 201–244 (A.R. Liss, New York, 1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nice, E. Micropreparative LC of proteins. Nature 348, 462–463 (1990). https://doi.org/10.1038/348462a0
Issue Date:
DOI: https://doi.org/10.1038/348462a0
This article is cited by
-
Strategies for the identification and purification of ligands for orphan biomolecules
Letters in Peptide Science (1997)