Abstract
CYCLINS, as subunits of the protein kinase encoded by the cdc2 gene are major controlling elements of the eukaryotic cell cycle1–3. The fission yeast Schizosaccharomyces pombe has a B-type cyclin, which is a nuclear protein7,8 encoded by the cdc13 gene4–6. Here we demonstrate the presence of two spatially distinct cdc13 cyclin populations in the nucleus of S. pombe, one of which is associated with the mitotic spindle poles. Both populations colocalize with the product of the cdc2 gene (p34cdc2). Treatment of cells with the antimicrotubule drug thiabendazole prevents cyclin degradation and blocks the tyrosine dephosphorylation and activation of cdc2. These results suggest a key regulatory role of the cdc2-cyclin complex in the initiation of mitotic spindle formation and also that mitotic microtubule function is required for cdc2 activation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Evans, T., Rosenthal, E. T., Youngbloom, J., Distel, D. & Hunt, T. Cell 33, 389–396 (1983).
Hunt, T. Curr. Opinions Cell Biol. 1, 268–274 (1989).
Draetta, G. et al. Cell 56, 829–838 (1989).
Booher, R. & Beach, D. EMBO J. 7, 2321–2327 (1988).
Solomon, M., Booher, R., Kirschner, M. & Beach, D. Cell 54, 738 (1988).
Hagan, I., Hayles, J. & Nurse, P. J. Cell Sci. 91, 587–595 (1988).
Booher, R. N., Alfa, C. E., Hyams, J. S. & Beach, D. H. Cell 58, 485–497 (1989).
Alfa, C. E., Booher, R., Beach, D. & Hyams, J. S. J. Cell Sci. Suppl. 12, 9–19 (1989).
King, S. M. & Hyams, J. S. Can. J. Microbiol. 28, 261–264 (1982).
Hagan, I. M. Thesis, Univ. London (1988).
Umesono, K., Hiraoka, Y., Toda, T. & Yanagida, M. Curr. Genet. 7, 123–128 (1983).
Davidse, L. C. A. Rev. Phytopathol. 24, 43–65 (1986).
Walker, G. M. J. gen. Microbiol. 128, 61–71 (1982).
Gould, K. L. & Nurse, P. Nature 342, 39–45 (1989).
Riabowol, K., Draetta, G., Brizuela, L., Vandre, D. & Beach, D. Cell 57, 393–401 (1989).
Bailly, E., Doree, M., Nurse, P. & Bornens, M. EMBO J. 8, 3985–3995 (1989).
Hyams, J. S. & Borisy, G. G. J. Cell Biol. 78, 401–414 (1978).
Hagan, I. M. & Hyams, J. S. J. Cell Sci. 89, 343–357 (1988).
Ducommun, B., Tollon, Y., Gares, M., Beach, D. & Wright, M. J. Cell Sci. 96, 683–689 (1990).
Draetta, G. & Beach, D. Cell 54, 17–26 (1988).
Moreno, S., Hayles, J. & Nurse, P. Cell 58, 361–372 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alfa, C., Ducommun, B., Beach, D. et al. Distinct nuclear and spindle pole body populations of cyclin–cdc2 in fission yeast. Nature 347, 680–682 (1990). https://doi.org/10.1038/347680a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/347680a0
This article is cited by
-
An updated view on the centrosome as a cell cycle regulator
Cell Division (2022)
-
Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast
Nature Cell Biology (2013)
-
Fission yeast Pcp1 links polo kinase-mediated mitotic entry to γ-tubulin-dependent spindle formation
The EMBO Journal (2010)
-
Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast
EMBO reports (2009)
-
Mitotic regulation by NIMA-related kinases
Cell Division (2007)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.