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Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells

Nature volume 345, pages 175–176 (1990)Cite this article

Abstract

INTRACELLULAR parasites can be classified into those that reside within a host vacuole and those which grow directly in the host cytoplasm1. Members of the latter group, which includes Rickettsia2, Shigellae3, Trypanosoma cruzi4, and Listeria monocytogenes5,6, possess haemolytic activity associated with the ability to enter the host cytoplasm5–7. Therefore mutants of L. monocytogenes lacking a pore-forming haemolysin, listeriolysin O, do not escape from the endosomal compartment5,6 and consequently fail to become established in the cytoplasm5,8,9. To examine the role of listeriolysin O, we cloned the structural gene for the L. monocytogenes haemolysin, hlyA, into an asporogenic mutant of Bacillus subtilis under the control of an IPTG-inducible promoter10. After being internalized by the macrophage-like cell line J774, haemolytic B. subtilis disrupted the phagosomal membrane and grew rapidly within the macrophage cytoplasm. These results show that a single gene product is sufficient to convert a common soil bacterium into a parasite that can grow in the cytoplasm of a mammalian cell.

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Author notes

  1. Patricia Connelly: Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018, USA

Authors and Affiliations

  1. Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104-6076, USA

    Jacek Bielecki, Philip Youngman, Patricia Connelly & Daniel A. Portnoy

  2. Institute of Microbiology Warsaw University Nowy Swiat, 67 00 046, Warsaw, Poland

    Jacek Bielecki

Authors
  1. Jacek Bielecki
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  2. Philip Youngman
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  3. Patricia Connelly
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  4. Daniel A. Portnoy
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Bielecki, J., Youngman, P., Connelly, P. et al. Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells. Nature 345, 175–176 (1990). https://doi.org/10.1038/345175a0

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