Abstract
Changes in intracellular free Ca2+ are involved in the transmembrane signalling of different cells, including lymphocytes1–3. Since calmodulin (CaM) is a primary receptor for Ca2+ (ref. 4), it may mediate the activation of crucial enzymes after antigen-induced increases in cytosolic Ca2+. Using a biotinylated-CaM (Bio-CaM) detection procedure5 to identify such proteins, we found that a peptide of relative molecular mass 59,000 (59K) was the predominant soluble CaM-binding protein (CaM-BP) in T cells and B lymphocytes from murine spleen; immunoblotting experiments identified it as a subunit of the CaM-dependent phosphatase, 'calcineurin' (CN)6. Smaller amounts of larger CaM-BPs, thought to be cytoskeletal-binding proteins, were also detected. CaM-BPs were expressed differentially, with B lymphocytes having four times more of the CN-like protein than T lymphocytes, while in thymocytes, a 65K polypeptide was the major CaM-BP. However, limited proteolysis analysis suggested that this thymus-specific peptide may be a precursor of CN. These data suggest that Ca2+-stimulated protein dephosphorylation may be an important and highly regulated function in lymphoid cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Allwood, G., Asherson, G. L., Davey, M. J. & Goodford, P. J. Immunology 21, 509-516 (1971). 2. Freedman, M. H. & Raff, M. C. Nature 255, 378-382 (1975). 3. Tsien, R. Y., Pozzan, T. & Rink, T. J. Nature 295, 68-71 (1982). 4. Means, A. R. & Dedman, J. R. Nature 285, 73-78 (1980). 5. Billingsley, M. L., Pennypacker, K. R., Hoover, C. G., Brigati, D. J. & Kincaid, R. L. Proc. natn. Acad. Sci. U.S.A. 82, 7585-7589 (1985). 6. Klee, C. B., Crouch, T. H. & Krinks, M. H. Proc. natn. Acad. Sci. U.S.A. 76,6270-6273 (1979). 7. Kincaid, R. L. & Vaughan, M. Proc. natn. Acad. Sci. U.S.A. 76, 4903-4907 (1979). 8. Sharma, R. K., Wang, T. H., Wirch, E. & Wang, J. H. J. biol. Chem. 255, 5916-5923 (1980). 9. Kincaid, R. L. et al. J. biol. Chem. 259, 5158-5166 (1984). 10. Bennett, M. K., Erondu, N. E. & Kennedy, M. B. /. biol. Chem. 258, 12735-12744 (1983). 11. Goldenring, J. R., Gonzalez, B., McGuire, J. S. Jr & DeLorenzo, R. J. /. biol. Chem. 258, 12632-12640 (1983). 12. Klee, C. B. & Krinks, M. H. Biochemistry 17, 120-126 (1978). 13. Wallace, R. W., Lynch, T. J., Tallant, E. A. & Cheung, W. Y. J. biol. Chem. 254, 377-382 (1979). 14. Sobue, K., Muramoto, V., Fujita, M. & Kakiuchi, S. Biochem. biophys. Res. Commun. 100, 1063-1070 (1981). 15. Sobue, K., Muramoto, Y., Fujita, M. & Kakiuchi, S. Proc. natn. Acad. Sci. U.S.A. 78, 5652-5655 (1981). 16. Bretscher, A. J. biol. Chem. 259, 12873-12880 (1984). 17. Dasch, J. R. & Stavitsky, A. B. Molec. Immun. 22, 379-389 (1985). 18. Harrison, M. L., Low, P. S. & Geahlen, R. L. /. biol. Chem. 259, 9348-9350 (1984). 19. Mishel, B. B. & Shiigi, S. M. (eds) in Selected Methods in Cellular Immunology (Freeman, San Francisco, 1980). 20. Bhattacharya, A., Dorf, M. E. & Sprinter, T. A. /. Immun. 127, 2488-2495 (1981). 21. Pallen, C. J. & Wang, J. H. Archs Biochem. Biophys. 237, 281-291 (1985). 22. Chantler, P. D. /. Cell Biol. 101, 207-216 (1985). 23. Wood, G. C., Wallace, R. W., Whitaker, G. N. & Cheung, W. Y. J. Cell Biol. 84,66-76 (1980). 24. Kincaid, R. L., Balaban, C. D. & Billingsley, M. L. Proc. natn. Acad. Sci. U.S.A. 84,1118-1122 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kincaid, R., Takayama, H., Billingsley, M. et al. Differential expression of calmodulin-binding proteins in B, T lymphocytes and thymocytes. Nature 330, 176–178 (1987). https://doi.org/10.1038/330176a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/330176a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.