Abstract
We report the isolation of a cosmid clone containing the entire human COL7A1 gene in one piece. The ability of the genomic sequences within this clone to direct tissue-specific expression of human collagen VII in transgenic mice was tested. The data show that the gene construct is capable of directing expression of collagen VII in the skin of fetal and neonatal transgenic mice. Expression of COL7A1 in these mice was widespread, in a pattern consistent with that found in human tissues and was in parallel with that of the endogenous mouse gene. Immunostaining, using human-specific antibodies, showed that human collagen VII protein was present at the skin basement membrane zone of the transgenic mice. Dermal extracts from 19-month-old transgenic mice contained mature human collagen VII protein, and fibroblasts derived from skin biopsies of these mice actively synthesized human collagen VII. The demonstration of successful and stable expression of human collagen VII in in vivo gene transfer is the first step towards the future development of therapeutic protocols for the rescue of keratinocyte function in severe blistering diseases such as dystrophic epidermolysis bullosa.
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Acknowledgements
This study was supported by research grants from the Dystrophic Epidermolysis Bullosa Research Association (DEBRA), The University of Hong Kong Committee for Research and Conference Grants, the Hong Kong Research Grants Council-DAAD Joint Research Scheme and the German Research Council (DFG, grants Br 1475/2–3 and SFB 492/A3). We are also very grateful for skilled technical assistance from Sandra YY Wong and Margit Schubert.
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Sat, E., Leung, K., Bruckner-Tuderman, L. et al. Tissue-specific expression and long-term deposition of human collagen VII in the skin of transgenic mice: implications for gene therapy. Gene Ther 7, 1631–1639 (2000). https://doi.org/10.1038/sj.gt.3301281
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DOI: https://doi.org/10.1038/sj.gt.3301281