Abstract
The yeast retrotransposon, Ty, encodes a set of proteins that are assembled into virus-like particles, Ty-VLPs (refs 1, 2). These proteins include Ty-VLP structural proteins, a protease that mediates cleavage of primary translation products and a reverse tran-scriptase1,3. The major structural components of Ty-VLPs are proteolytic products of the primary translation product, pl (ref. 3). We have recently shown that protein pi alone can form Ty-VLPs (ref. 3). Here we demonstrate that pl fusion proteins, comprising most of pi and part of human immunodeficiency virus (HIV) protein gp120, form hybrid HIV:Ty-VLPs. These hybrid particles provide a rapid means of preparing and evaluating HIV antigens for a variety of immunological purposes.
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Adams, S., Dawson, K., Gull, K. et al. The expression of hybrid HIV:Ty virus-like particles in yeast. Nature 329, 68–70 (1987). https://doi.org/10.1038/329068a0
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DOI: https://doi.org/10.1038/329068a0
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