Abstract
A germline T-cell receptor variable region (Vβ) gene segment (Vβ14) has been mapped 10 kilobases to the 3′ side of the constant region (Cβ2) gene. The Vβ14 gene segment is in an inverted transcriptional polarity relative to the diversity-region (Dβ) and joining-region (Jβ) gene segments and the Cβ genes. Analyses of a T-cell clone (J6.19), which has productively rearranged the Vβ14 gene segment, indicate that the productive Vβ-Dβ-Jβ rearrangement and its reciprocal flank recombination product are linked and located at either border of a chromosomal inversion. These data demonstrate for the first time a linkage between mammalian V and C genes and verify that a functional T-cell receptor Vβ gene can be constructed through a chromosomal inversion.
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Malissen, M., McCoy, C., Blanc, D. et al. Direct evidence for chromosomal inversion during T-cell receptor β-gene rearrangements. Nature 319, 28–33 (1986). https://doi.org/10.1038/319028a0
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DOI: https://doi.org/10.1038/319028a0
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