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Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

Nature volume 313, pages 762–766 (1985)Cite this article

Abstract

The 3.3-Å resolution crystal structure of the large proteolytic fragment of Escherichia coli DNA polymerase I complexed with deoxythymidine monophosphate consists of two domains, the smaller of which binds zinc-deoxythymidine monophosphate. The most striking feature of the larger domain is a deep crevice of the appropriate size and shape for binding double-stranded B-DNA. A flexible subdomain may allow the enzyme to surround completely the DNA substrate, thereby allowing processive nucleotide polymerization without enzyme dissociation.

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References

  1. Kornberg, A. DNA Replication (Freeman, San Fransisco, 1980).

    Google Scholar 

  2. Klenow, H. & Henningsen, I. Proc. natn. Acad. Sci. U.S.A. 65, 168–175 (1970).

    Article  ADS  CAS  Google Scholar 

  3. Brutlag, D., Atkinson, M. R., Setlro, P. & Kornberg, A. Biochem. biophys Res. Commun. 37, 982–989 (1969).

    Article  CAS  Google Scholar 

  4. Springate, C. F., Mildvan, A. S., Abramson, R., Engle, J. L. & Loeb, L. A. J. biol. Chem. 248, 5987–5993 (1973).

    Google Scholar 

  5. Walton, K. E., Fitzgerald, P. C., Herrman, M. S. & Behnke, W. D. Biochem. biophys. Res. Commun. 108, 1353–1368 (1982).

    Article  CAS  Google Scholar 

  6. Que, B. G., Downey, K. M. & So, A. Biochemistry 17, 1603–1606 (1978).

    Article  CAS  Google Scholar 

  7. Huberman, J. A. & Kornberg, A. J. biol. Chem. 245, 5326–5337 (1970).

    CAS  PubMed  Google Scholar 

  8. Englund, P. T., Huberman, J. A., Jovin, T. M. & Kornberg, A. J. biol. Chem. 244, 3038–3049 (1969).

    CAS  PubMed  Google Scholar 

  9. Joyce, C. M. & Grindley, N. D. F. Proc. natn. Acad. Sci. U.S.A. 80, 1830–1834 (1983).

    Article  ADS  CAS  Google Scholar 

  10. Brick, P., Ollis, D. & Steitz, T. A. J. molec. Biol. 166, 453–456 (1983).

    Article  CAS  Google Scholar 

  11. Steitz, T. A., Weber, I. T., Ollis, D. & Brick, P. J. biomolec. Struct. Dyn. 1, 1023–1037 (1983).

    Article  CAS  Google Scholar 

  12. Joyce, C. M., Kelley, W. S. & Grindley, N. D. F. J. biol. Chem. 257, 1958–1964 (1982).

    CAS  Google Scholar 

  13. Xuong, N. H., Freer, S. T., Hamlin, R., Neilsen, C. & Vernon, W. Acta crystallogr. A34, 289–296 (1978).

    Article  Google Scholar 

  14. Jones, A. T. J. appl. Crysallogr. 11, 268–272 (1978).

    Article  CAS  Google Scholar 

  15. Hendrickson, W. A. & Konnert, J. H. in Computing in Crystallography (eds Diamond, R., Ramasehan, S. & Venkatesan, K.) 1301–1323 (Indian Acad. of Science for Internat. Union of Crystallography, 1980).

    Google Scholar 

  16. Remington, J., Wiegand, G. & Huber, R. J. molec. Biol. 158, 111–152 (1982).

    Article  CAS  Google Scholar 

  17. Steitz, T. A., Fletterick, R. J., Anderson, W. F. & Anderson, C. M. J. molec. Biol. 104, 197–222 (1976).

    Article  CAS  Google Scholar 

  18. Liljas, A. & Rossmann, M. G. A. Rev. Biochem. 43, 475–508 (1974).

    Article  CAS  Google Scholar 

  19. Cotton, F. A., Hazen, E. A. & Legg, M. J. Proc. natn. Acad. Sci. U.S.A. 76, 2551–2555 (1979).

    Article  ADS  CAS  Google Scholar 

  20. Steitz, T. A., Ohlendorf, D. H., McKay, D. B., Anderson, W. F. & Matthews, B. W. Proc. natn. Acad. Sci. U.S.A. 79, 3097–3100 (1982).

    Article  ADS  CAS  Google Scholar 

  21. Mckay, D. B., Weber, I. T. & Steitz, T. A. J. biol. Chem. 257, 9418–9524 (1982).

    Google Scholar 

  22. Anderson, W. F., Ohlendorf, D. H., Takeda, Y. & Matthews, B. W. Nature 290, 754–758 (1981).

    Article  ADS  CAS  Google Scholar 

  23. Pabo, C. O. & Lewis, M. Nature 298, 443–447 (1982).

    Article  ADS  CAS  Google Scholar 

  24. Das, S. K. & Fujimura, R. K. J. biol. Chem. 254, 1227–1232 (1979).

    CAS  PubMed  Google Scholar 

  25. Fairfield, F. R., Newport, J. W., Dolejsi, M. K. & von Hippel, P. H. J. biomolec. Struct. Dyn. 1, 715–727 (1983).

    Article  CAS  Google Scholar 

  26. Kelly, W. S. & Grindley, ND. F. Nucleic Acids Res. 3, 2971–2984 (1976).

    Article  CAS  Google Scholar 

  27. Matson, S. W., Capaldo-Kimball, F. N. & Bambara, R. A. J. biol. Chem. 253, 7851–7856 (1968).

    Google Scholar 

  28. Ollis, D., Kline, C. & Steitz, T. A. Nature this issue.

  29. Bennett, W. S. & Steitz, T. A. Proc. natn. Acad. Sci. U.S.A. 75, 4848–4852 (1978).

    Article  ADS  CAS  Google Scholar 

  30. Lee, B. & Richards, F. M. J. Molec. Biol. 55, 379–389 (1971).

    Article  CAS  Google Scholar 

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Author notes

  1. P. Brick

    Present address: Biophysics Group, Imperial College, London, SW7 2AZ, UK

Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06511, USA

    D. L. Ollis, P. Brick & T. A. Steitz

  2. Department of Physics, University of California, La Jolla, California, 92093, USA

    R. Hamlin & N. G. Xuong

Authors
  1. D. L. Ollis
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  2. P. Brick
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  3. R. Hamlin
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  4. N. G. Xuong
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  5. T. A. Steitz
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Ollis, D., Brick, P., Hamlin, R. et al. Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP. Nature 313, 762–766 (1985). https://doi.org/10.1038/313762a0

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