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Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells

Nature volume 313, pages 191–196 (1985)Cite this article

Abstract

Co-cultivation of Agrobacterium tumefaciens with plant cells leads to the induction of circular copies of the T-DNA segment of the large tumour-inducing (Ti) plasmid in the bacterial cells. These circular molecules are presumably intermediates in DNA transfer from the A. tumefaciens genome to the plant cells. In support of this suggestion, the junction of the T-DNA circles occurs precisely in the 25-base pair terminal sequence involved in T-DNA transfer.

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Authors and Affiliations

  1. Friedrich-Miescher Institut, PO Box 2543, CH-4002, Basel, Switzerland

    Zdena Koukolíková-Nicola, Raymond D. Shillito & Barbara Hohn

  2. Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000, Gent, Belgium

    Kan Wang, Marc Van Montagu & Patricia Zembryski

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  1. Zdena Koukolíková-Nicola
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  2. Raymond D. Shillito
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  3. Barbara Hohn
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  4. Kan Wang
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  5. Marc Van Montagu
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  6. Patricia Zembryski
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Koukolíková-Nicola, Z., Shillito, R., Hohn, B. et al. Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells. Nature 313, 191–196 (1985). https://doi.org/10.1038/313191a0

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