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Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage λ

Nature volume 311, pages 721–726 (1984)Cite this article

Abstract

Site-specific recombination of the bacteriophage λ genome into and out of the host bacterial genome is postulated to involve the formation of Holliday structure intermediates by reciprocal single-strand exchanges. Synthetic analogues of the predicted recombination intermediates are resolved in vitro by the protein product of the λ int gene. Some of the structural features and reaction conditions for this genetic recombination can now be defined.

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References

  1. Nash, H. A. A. Rev. Genet. 15, 143–167 (1981).

    Article  CAS  Google Scholar 

  2. Weisberg, R. & Landy, A. in Lambda II (eds Hendrix, R. W., Roberts, J. W., Stahl, F. W. & Weisberg, R. W.) 211–250 (Cold Spring Harbor Laboratory, New York, 1983).

    Google Scholar 

  3. Kikuchi, A. & Nash, H. A. Proc. natn. Acad. Sci. U.S.A. 76, 3760–3764 (1979).

    Article  ADS  CAS  Google Scholar 

  4. Nash, H. A. & Robertson, C. A. J. biol. Chem. 256, 9246–9253 (1981).

    CAS  PubMed  Google Scholar 

  5. Abremski, K. & Gottesman, S. J. biol. Chem. 257, 9658–9710 (1982).

    CAS  PubMed  Google Scholar 

  6. Better, M., Wickner, S., Auerback, J., Williams, R. & Echols, H. Cell 32, 161–168 (1983).

    Article  CAS  Google Scholar 

  7. Hsu, P-L., Ross, W. & Landy, A. Nature 285, 85–91 (1980).

    Article  ADS  CAS  Google Scholar 

  8. Landy, A. & Ross, W. Science 197, 1147–1160 (1977).

    Article  ADS  CAS  Google Scholar 

  9. Ross, W. & Landy, A. Proc. natn. Acad. Sci. U.S.A. 79, 7724–7728 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Ross, W. & Landy, A. Cell 33, 261–272 (1983).

    Article  CAS  Google Scholar 

  11. Mizuuchi, M. & Mizuuchi, K. Proc. natn. Acad. Sci. U.S.A. 77, 3220–3224 (1980).

    Article  ADS  CAS  Google Scholar 

  12. Mizuuchi, K. et al. Cold Spring Harb. Symp. quant. Biol. 45, 429–437 (1981).

    Article  CAS  Google Scholar 

  13. Weisberg, R., Enquist, L., Foeller, C. & Landy, A. J. molec. Biol. 170, 319–342 (1983).

    Article  CAS  Google Scholar 

  14. Enquist, L. W., Nash, H. A. & Weisberg, R. A. Proc. natn. Acad. Sci. U.S.A. 76, 1363–1367 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Echols, H. & Green, L. Genetics 93, 297–307 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Holliday, R. Genet. Res. 5, 282–304 (1964).

    Article  Google Scholar 

  17. Sigal, N. & Alberts, B. J. molec. Biol. 71, 789–793 (1972).

    Article  CAS  Google Scholar 

  18. Holliday, R. Genetics 78, 273–287 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Dressler, E. & Potter, H. A. Rev. Biochem. 51, 727–761 (1982).

    Article  CAS  Google Scholar 

  20. Stahl, F. Genetic Recombination. Thinking about it in Phage and Fungi (Freeman, San Francisco, 1979).

    Google Scholar 

  21. Thompson, B. et al. J. molec. Biol. 91, 409–419 (1975).

    Article  CAS  Google Scholar 

  22. Benbow, R., Zuccavelli, A. & Sinsheimer, R. Proc. natn. Acad. Sci. U.S.A. 72, 235–239 (1975).

    Article  ADS  CAS  Google Scholar 

  23. Valenzuela, M. & Inman, R. Proc. natn. Acad. Sci. U.S.A. 72, 3024–3028 (1975).

    Article  ADS  CAS  Google Scholar 

  24. Thompson, B. J., Camien, M. N. & Warner, R. C. Proc. natn. Acad. Sci. U.S.A. 73, 2299–2303 (1976).

    Article  ADS  CAS  Google Scholar 

  25. Potter, H. & Dressler, D. Proc. natn. Acad. Sci. U.S.A. 73, 3000–3004 (1976).

    Article  ADS  CAS  Google Scholar 

  26. Bell, L. & Byers, B. Proc. natn. Acad. Sci. U.S.A. 76, 3445–3449 (1979).

    Article  ADS  CAS  Google Scholar 

  27. Wolgemuth, D. J. & Hsu, M. T. Nature 287, 168–171 (1980).

    Article  ADS  CAS  Google Scholar 

  28. Cozzarelli, N. R. Science 207, 953–960 (1980).

    Article  ADS  CAS  Google Scholar 

  29. Gellert, M. A. Rev. Biochem. 50, 879–910 (1981).

    Article  CAS  Google Scholar 

  30. Liu, L. F. & Miller, K. G. Proc. natn. Acad. Sci. U.S.A. 78, 3487–3491 (1981).

    Article  ADS  CAS  Google Scholar 

  31. Liu, L. F., Liu, C.-C. & Alberts, B. M. Nature 281, 456–461 (1979).

    Article  ADS  CAS  Google Scholar 

  32. Miller, K. G., Liu, L. F. & Englund, P. T. J. biol. Chem. 256, 9334–9339 (1981).

    CAS  PubMed  Google Scholar 

  33. Miller, H. I. & Friedman, D. I. Cell 20, 711–719 (1980).

    Article  CAS  Google Scholar 

  34. Nash, H. A., Mizuuchi, K., Enquist, L. W. & Weisberg, R. A. Cold Spring Harb. Symp. quant. Biol. 45, 417–428 (1981).

    Article  CAS  Google Scholar 

  35. Craig, N. & Nash, H. Cell 35, 795–803 (1983).

    Article  CAS  Google Scholar 

  36. Mizuuchi, K., Kemper, B., Hays, J. & Weisberg, R. Cell 29, 357–365 (1982).

    Article  CAS  Google Scholar 

  37. Hamilton, P., Yuan, R. & Kikuchi, Y. J. molec. Biol. 152, 163–170 (1981).

    Article  CAS  Google Scholar 

  38. Better, M., Chi, L., Williams, R. C. & Echols, H. Proc. natn. Acad. Sci. U.S.A. 79, 5837–5841 (1982).

    Article  ADS  CAS  Google Scholar 

  39. Davies, R. W., Schreier, P. H., Kotewicz, M. L. & Echols, H. Nucleic Acids Res. 7, 2255–2273 (1979).

    Article  CAS  Google Scholar 

  40. Nash, H. & Pollock, T. J. molec. Biol. 170, 19–38 (1983).

    Article  CAS  Google Scholar 

  41. Pollock, T. & Nash, H. J. molec. Biol. 170, 1–18 (1983).

    Article  CAS  Google Scholar 

  42. Rossi, J. J., Ross, W., Egan, J., Lipman, D. J. & Landy, A. J. molec. Biol. 128, 21–47 (1979).

    Article  CAS  Google Scholar 

  43. Nash, H. A. Meth. Enzym. 100, 210–216 (1983).

    Article  CAS  Google Scholar 

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Author notes

  1. Pei Ling Hsu

    Present address: Department of Pathology, University of Texas Health Science Center, Houston, Texas, 77225, USA

Authors and Affiliations

  1. Division of Biology and Medicine, Brown University, Providence, Rhode Island, 02912, USA

    Pei Ling Hsu & Arthur Landy

Authors
  1. Pei Ling Hsu
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  2. Arthur Landy
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Hsu, P., Landy, A. Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage λ. Nature 311, 721–726 (1984). https://doi.org/10.1038/311721a0

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  • DOI: https://doi.org/10.1038/311721a0

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