Abstract
During receptor-mediated phagocytosis, macrophages release toxic molecules such as hydrogen peroxide which enable them1 to kill antibody-coated tumour cells2 and parasites3, too large to consume. Previous workers observed that while peroxide was clearly responsible for cytolysis of certain antibody-coated tumour cells2,4,5, extracellular catalase was unable to inhibit this cytolysis, and they suggested that macrophages secrete peroxide into a protected cleft between the phagocyte and target. We have tested this and report here that the space beneath macrophages spread on glass surfaces is accessible to proteins with a molecular weight (MW) as large as 200,000 but the space beneath macrophages plated on glass surfaces coated with phagocytosis-promoting ligands is impermeable to proteins as small as 50,000 MW. It appears indeed that macrophages form a protein-tight seal at the periphery of their contact with ligand-coated surfaces and thereby create a closed compartment between the cell and the target.
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Wright, S., Silverstein, S. Phagocytosing macrophages exclude proteins from the zones of contact with opsonized targets. Nature 309, 359–361 (1984). https://doi.org/10.1038/309359a0
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DOI: https://doi.org/10.1038/309359a0
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