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Monoclonal antibodies distinguish between storage and secreted forms of eosinophil cationic protein

Nature volume 309, pages 182–184 (1984)Cite this article

Abstract

The toxic effects of eosinophils on parasites1 and cells2 are due largely to the secretion of various granule proteins, following stimulation3. In order to study this secretory process (degranulation) further, we have raised mouse monoclonal antibodies against both human eosinophil granule extracts and secretion products. From immunocytochemical studies it appears that one antibody, EG1, recognized both the storage and secreted forms of eosinophilcationic protein (ECP), whereas antibody EG2 only bound to ECP during secretion (and extraction). This antibody also bound to eosinophil protein-X (EP-X). As both antibodies stained eosinophils in formalin-fixed tissues, they were used to demonstrate sites of eosinophil activation and secretion in chronic urticaria. The capacity of monoclonal antibodies to detect differences between storage and secreted forms of proteins is an important property of these reagents with many potential applications in cell biology.

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Author notes

  1. Christer Peterson and Per Venge: Department of Clinical Chemistry, University Hospital, S-751 85, Uppsala, Sweden

  2. Inge Olsson: Research Department 2, University Hospital, S-221 85, Lund, Sweden

Authors and Affiliations

  1. Department of Immunology, Royal Postgraduate Medical School, London, W12 0HS, UK

    Po-Chun Tai, Christopher J. F. Spry, Christer Peterson, Per Venge & Inge Olsson

Authors
  1. Po-Chun Tai
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  2. Christopher J. F. Spry
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  3. Christer Peterson
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  4. Per Venge
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  5. Inge Olsson
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Tai, PC., Spry, C., Peterson, C. et al. Monoclonal antibodies distinguish between storage and secreted forms of eosinophil cationic protein. Nature 309, 182–184 (1984). https://doi.org/10.1038/309182a0

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