Abstract
There is considerable interest in the existence and significance of alternative conformations of DNA1–4 to the right-handed B-form described originally by Watson and Crick5. The indirect immunofluorescence observations of Nordheim et al.6, Arndt-Jovin et al.7 and Lemeunier et al.8 that antibodies against left-handed Z-DNA bind to polytene chromosomes have thus assumed considerable importance. However, there is a paradox: some workers observe Z-DNA in interbands and others in bands. We report here that binding of Z-DNA antibodies to Drosophila polytene chromosomes prepared without acid fixation is at background level, and that following acid fixation the same antibody treatment leads to intense fluorescence. Depending on the extent of exposure to 45% acetic acid, fluorescence can occur primarily in interbands or in bands. Furthermore, antibody binding is dependent on elastic torsional strain in the DNA molecules.
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References
Wang, A. H.-J. et al. Nature 282, 680–686 (1979).
Wells, R. D. et al. Prog. Nucleic Acid Res. molec. Biol. 24, 167–267 (1980).
Cantor, C. R. Cell 25, 293–295 (1981).
Dickerson, R. E. et al. Science 216, 475–485 (1982).
Watson, J. & Crick, F. H. C. Nature 171, 737 (1953).
Nordheim, A. et al. Nature 294, 417–422 (1981).
Arndt-Jovin, D. J. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Lemeunier, F., Derbin, C., Malfoy, B., Leng, M. & Taillandier, E. Expl Cell Res. 141, 508–513 (1982).
Painter, T. S. Genetics 19, 175–188 (1934).
Hill, R. J. & Watt, F. Cold Spring Harb. Symp. quant. Biol. 42, 859–865 (1978).
D'Angelo, E. G. Biol. Bull. 90, 71–87 (1946).
Silver, L. M. & Elgin, S. C. R. in The Cell Nucleus Vol. 5 (ed. Busch, H.) 215–262 (Academic, New York, 1978).
Hewish, D. R. & Burgoyne, L. A. Biochem. biophys. Res. Commun. 52, 504–510 (1973).
Mott, M. R., Burnett, E. J. & Hill, R. J. J. Cell Sci. 45, 15–30 (1980).
Hill, R. J. et al. J. Cell Biol. 95, 262–266 (1982).
Lafer, E. M., Moller, A., Nordheim, A., Stollar, B. D. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 78, 3546–3550 (1981).
Nordheim, A. et al. Proc. natn. Acad. Sci. U.S.A. 79, 7729–7733 (1982).
Dick, C. & Johns, E. W. Expl Cell Res. 51, 626–632 (1968).
Germond, J. E., Hint, B., Oudet, P., Gross-Bellard, M. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 72, 1843–1847 (1975).
Singleton, C. K., Klysik, J., Stirdivant, S. M. & Wells, R. D. Nature 299, 312–316 (1982).
Peck, L. J., Nordheim, A., Rich, A. & Wang, J. C. Proc. natn. Acad. Sci. U.S.A. 79, 4560–4564 (1982).
Nordheim, A. et al. Cell 31, 309–318 (1982).
Hamada, H., Petrino, M. G. & Kakunaga, T. Proc. natn. Acad. Sci. U.S.A. 79, 6465–6469 (1982).
Haniford, D. B. & Pulleyblank, D. E. Nature 302, 632–634 (1983).
Nordheim, A. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 80, 1821–1825 (1983).
Guschlbaner, W. & Courtois, Y. FEBS Lett. 1, 183–186 (1968).
Jordan, D. O. The Chemistry of Nucleic Acids, 206–216 (Butterworths, London, 1960).
Nickol, J., Behe, M. & Felsenfeld, G. Proc. natn. Acad. Sci. U.S.A. 79, 1771–1775 (1982).
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Hill, R., Stollar, B. Dependence of Z-DNA antibody binding to polytene chromosomes on acid fixation and DNA torsional strain. Nature 305, 338–340 (1983). https://doi.org/10.1038/305338a0
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DOI: https://doi.org/10.1038/305338a0
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