Abstract
Repair of DNA damage in eukaryotic cells involves local disruption of chromatin structure such that repair-incorporated nucleotides are transiently highly sensitive to digestion by staphylococcal nuclease (SN)1–4. We have examined the dependence of this phenomenon on the initial distribution in chromatin of DNA damage by comparing the repair of pyrimidine dimers, which are distributed randomly in chromatin5, with the repair of angelicin photoadducts, which are formed primarily in linker regions of nucleosomes6,7. We find that the transient nuclease sensitivity of repair patches is independent of the initial distribution of DNA damage in chromatin. In addition, our observation that repair patches produced in response to a linker-specific damaging agent become distributed randomly in chromatin implies that nucleosome cores do not necessarily return to their original sites along a DNA strand after the DNA is repaired.
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References
Smerdon, M. J. & Lieberman, M. W. Proc. natn. Acad. Sci. U.S.A. 75, 4238–4241 (1978).
Bodell, W. J. & Cleaver, J. E. Nucleic Acids Res. 9, 203–212 (1981).
Oleson, F. B., Mitchell, B. L., Dipple, A. & Lieberman, M. W. Nucleic Acids Res. 7, 1343–1361 (1979).
Lieberman, M. W., Smerdon, M. J., Tlsty, T. D. & Oleson, F. B. in Environmental Carcinogenesis (eds Emmelot, P. & Kriek, E.) 345–363 (Elsevier, Amsterdam, 1979).
Williams, J. I. & Friedberg, E. C. Biochemistry 18, 3965–3972 (1979).
Wiesenhahn, G. P., Hyde, J. E. & Hearst, J. E. Biochemistry 16, 925–932 (1979).
Cech, T. & Pardue, M. L. Cell 11, 631–640 (1977).
Dall'Acqua, F., Marciani, S., Ciavatta, L. & Rodighiero, G. Z. Naturf. 26 b, 561–569 (1971).
Kaye, J., Smith, C. A. & Hanawalt, P. C. Cancer Res. 40, 696–702 (1980).
Niggli, H. J. & Cerutti, P. A. Biochem. biophys. Res. Commun. 105, 1215–1223 (1982).
Stein, G. H. J. cell. Physiol. 99, 43–54 (1979).
Zolan, M. E., Cortopassi, G. A., Smith, C. A. & Hanawalt, P. C. Cell 28, 613–619 (1982).
Smerdon, M. J., Tlsty, T. D. & Lieberman, M. W. Biochemistry 17, 2377–2386 (1978).
Kornberg, R. D. A. Rev. Biochem. 46, 931–954 (1977).
Baer, B. W. & Kornberg, R. D. J. biol. Chem. 254, 9678–9681 (1979).
Prunnel, A. in Chromatin Structure and Function (ed. Nicolini, C.) 441–449 (Plenum, New York, 1979).
Nelson, P. P., Albright, S. C. & Garrad, W. T. J. biol. Chem. 254, 9194–9199 (1979).
Louis, C., Schedl, P., Samal, B. & Worcel, A. Cell 22, 387–392 (1980).
Wittig, B. & Wittig, S. Cell 18, 1173–1183 (1979).
Gottesfeld, J. M. & Bloomer, L. S. Cell 21, 751–760 (1980).
Igo-Kemenes, T., Omori, A. & Zachau, H. G. Nucleic Acids Res. 8, 5377–5390 (1980).
Cook, K. H. & Friedberg, E. C. Analyt. Biochem. 73, 411–418 (1976).
Smith, C. A., Cooper, P. K. & Hanawalt, P. C. in DNA Repair: A Laboratory Manual of Research Procedures Vol. 1 (eds Friedberg, E. C. & Hanawalt, P. C.) 289–305 (Dekker, New York, 1981).
Axel, R. Biochemistry 14, 2921–2925 (1975).
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Zolan, M., Smith, C., Calvin, N. et al. Rearrangement of mammalian chromatin structure following excision repair. Nature 299, 462–464 (1982). https://doi.org/10.1038/299462a0
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DOI: https://doi.org/10.1038/299462a0
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