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Structural and biological properties of a monoclonal auto-anti-(anti-idiotype) antibody

Nature volume 299, pages 447–449 (1982)Cite this article

Abstract

Several studies illustrating idiotype network-directed regulation of the immune response1 have demonstrated that heterologous anti-(anti-idiotype) antibodies (1) share variable (V) region antigenic determinants with idiotype-bearing antibodies2–5, (2) lack antigen-binding activity3–6 and (3) alter the clonal profile of an immune response by stimulating/suppressing regulatory lymphocyte clones of anti-idiotype specificity2–4,6. However, the existence and significance of anti-(anti-idiotype) antibody in natural immunological responses has been questioned because of the artificial immunization procedures used to induce these antibodies. Here we describe a monoclonal mouse anti-(anti-idiotype) antibody generated in physiological conditions by immunizing BALB/c mice with a pneumococcal vaccine that elicits a humoral anti-phosphorylcholine (PC) response dominated by the T15 idiotype7. This antibody recognized an idiotope on a monoclonal anti-T15 antibody, failed to bind PC, did not exhibit detectable idiotypic cross-reactivity with T15+ immunoglobulins, utilized VH and VL genes distinct from the T15 V gene group8, and exerted a suppressive effect on the in vivo response to PC in syngeneic mice.

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References

  1. Jerne, N. K. Annls Immun. Inst. Pasteur, Paris 125C, 373–389 (1974).

    CAS  Google Scholar 

  2. Cazenave, P. A. Proc. natn. Acad. Sci. U.S.A. 74, 5122–5125 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Urbain, J., Wilkler, M., Fransen, J. D. & Collignon, C. Proc. natn. Acad. Sci. U.S.A. 74, 5126–5130 (1977).

    Article  ADS  CAS  Google Scholar 

  4. Bona, C., Heber-Katz, E. & Paul, W. J. exp. Med. 153, 951–967 (1981).

    Article  CAS  Google Scholar 

  5. Wikler, M. et al. J. exp. Med. 150, 184–195 (1979).

    Article  Google Scholar 

  6. Bona, C., Hooghe, R., Cazenave, P., Leguern, C. & Paul, W. J. exp. Med. 149, 815–823 (1979).

    Article  CAS  Google Scholar 

  7. Sher, A. & Cohn, M. Eur. J. Immun. 2, 319–326 (1972).

    Article  CAS  Google Scholar 

  8. Crews, S., Griffin, J., Huang, H., Calame, K. & Hood, L. Cell 25, 59–66 (1981).

    Article  CAS  Google Scholar 

  9. Kearney, J., Radbruch, A., Liesegang, B. & Rajewsky, K. J. Immun. 123, 1548–1550 (1979).

    CAS  PubMed  Google Scholar 

  10. Kearney, J., Barletta, R., Quan, Z. & Quintans, J. Eur. J. Immun. 11, 877–882 (1981).

    Article  CAS  Google Scholar 

  11. Volanakis, J. & Kearney, J. J. exp. Med. 153, 1604–1614 (1981).

    Article  CAS  Google Scholar 

  12. Cook, W. D., Rudikoff, S., Giusti, A. M. & Scharff, M. D. Proc. natn. Acad. Sci. U.S.A. 79, 1240–1244 (1982).

    Article  ADS  CAS  Google Scholar 

  13. Rudikoff, S., Giusti, A. M., Cook, W. D. & Scharff, M. D. Proc. natn. Acad. Sci. U.S.A. 79, 1979–1983 (1982).

    Article  ADS  CAS  Google Scholar 

  14. Claflin, J. L. Eur. J. Immun. 6, 666–668 (1976).

    Article  CAS  Google Scholar 

  15. Potter, M. Adv. Immun. 25, 141–211 (1977).

    Article  CAS  Google Scholar 

  16. Gearhart, P. J., Johnson, N. D., Douglas, R. & Hood, L. Nature 291, 29–34 (1981).

    Article  ADS  CAS  Google Scholar 

  17. Cosenza, H., Julius, M. & Augustin, A. Eur. J. Immun. 6, 114–116 (1976).

    Article  CAS  Google Scholar 

  18. Kim, B. S. & Greenberg, J. J. exp. Med. 154, 809–820 (1981).

    Article  CAS  Google Scholar 

  19. Cosenza, H., Julius, M. & Augustin, A. Immun. Rev. 34, 3–33 (1977).

    Article  CAS  Google Scholar 

  20. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  21. Lonai, P., Ben-Neriah, Y., Steinman, L. & Givol, D. Eur. J. Immun. 8, 827–832 (1978).

    Article  CAS  Google Scholar 

  22. Sy, M-S. et al. Proc. natn. Acad. Sci. U.S.A. 78, 1143–1147 (1981).

    Article  ADS  CAS  Google Scholar 

  23. Slack, J., Der-Balian, G., Nahm, M. & Davie, J. J. exp. Med. 151, 853–862 (1980).

    Article  CAS  Google Scholar 

  24. Chesebro, B. & Metzgar, H. Biochemistry 11, 760–771 (1972).

    Article  Google Scholar 

  25. Kubagawa, H. et al. J. exp. Med. 150, 792–807 (1979).

    Article  CAS  Google Scholar 

  26. Bhown, A. S. et al. Analyt. Biochem. 102, 35–38 (1980).

    Article  CAS  Google Scholar 

  27. Barstad, P. et al. Science 183, 962–964 (1944).

    Article  ADS  Google Scholar 

  28. Kabat, E. A., Wu, T. & Bilofsky, H. Sequences of Immunoglobulin Chains (U.S. Department of Health, Education and Welfare, 1979).

    Google Scholar 

  29. Hämmerling, G. & Wallich, R. in Protides of the Biological Fluids, Vol. 28 (ed. Peeters, H.) 569–574 (Pergamon, Oxford, 1980).

    Google Scholar 

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Authors and Affiliations

  1. The Cellular Immunobiology Unit of the Tumor Institute, Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama, 35294, USA

    Brian A. Pollok & John F. Kearney

  2. The Comprehensive Cancer Center, University of Alabama in Birmingham, Birmingham, Alabama, 35294, USA

    Brian A. Pollok, Ajit S. Bhown & John F. Kearney

  3. Clinical Immunology and Rheumatology, University of Alabama in Birmingham, Birmingham, Alabama, 35294, USA

    Ajit S. Bhown

Authors
  1. Brian A. Pollok
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  2. Ajit S. Bhown
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  3. John F. Kearney
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Pollok, B., Bhown, A. & Kearney, J. Structural and biological properties of a monoclonal auto-anti-(anti-idiotype) antibody. Nature 299, 447–449 (1982). https://doi.org/10.1038/299447a0

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