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LexA protein inhibits transcription of the E. coli uvrA gene in vitro

Nature volume 298, pages 96–98 (1982)Cite this article

Abstract

Treatment of Escherichia coli with radiation or chemical carcinogens induces a number of cellular functions to counteract the damage inflicted by such treatments (see ref. 1 for a review). One of these inducible functions is the SOS response, which is controlled by the recA and lexA genes: single-stranded DNA produced as a result of DNA damage binds to recA protein (RecA), activating its protease function; activated RecA cuts lexA protein (LexA), the represser of the SOS genes, thereby inactivating it and turning on these genes2–4. The SOS genes include recA and lexA themselves, and recent work has shown that the two genes have similar operators where LexA binds in vitro, inhibiting their transcription3,4. It is anticipated that other genes under the control of recA and lexA will have similar operators. Recent genetic5,6 and biochemical7 data suggest that the excision repair genes uvrA and uvrB are part of the recA–lexA regulon and are at least partly responsible for recA–lexA-mediated inducible DNA repair. In support of these results we have reported that uvrB has a promoter that is repressed by LexA in vitro8. We now show that uvrA has an operator similar to those of other SOS genes and that LexA binds to this operator and inhibits the transcription of the uvrA gene in vitro.

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Authors and Affiliations

  1. Departments of Therapeutic Radiology and of Molecular Biophysics & Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510, USA

    Aziz Sancar, Gwendolyn B. Sancar & W. Dean Rupp

  2. Departments of Therapeutic Radiology and of Molecular Biophysics & Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510, USA

    John W. Little & David W. Mount

Authors
  1. Aziz Sancar
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  2. Gwendolyn B. Sancar
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  3. W. Dean Rupp
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  4. John W. Little
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  5. David W. Mount
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Sancar, A., Sancar, G., Rupp, W. et al. LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature 298, 96–98 (1982). https://doi.org/10.1038/298096a0

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