Abstract
Normal chicken embryo fibroblasts contain a gene, c-src, which is homologous to the avian sarcoma virus transforming gene, src1. Both genes code for a 60,000-molecular weight (Mr) protein which is phosphorylated at serine and tyrosine residues2–11 and has the unusual function of phosphorylating tyrosine residues10,11. We report here that both normal human fibroblasts and A431 human epidermoid carcinoma cells contain two forms of the normal cell protein pp60c-Src which were resolved electrophoretically using a 10% polyacrylamide gel. The 60,000-Mr form is phosphorylated primarily at tyrosine, while the 59,000-Mr form is phosphorylated at serine. 35S-methionine chymotryptic maps show that the two forms are clearly related, although several significant differences are observed.
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Shealy, D., Erikson, R. Human cells contain two forms of pp60c-src. Nature 293, 666–669 (1981). https://doi.org/10.1038/293666a0
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DOI: https://doi.org/10.1038/293666a0
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