Abstract
Previous research in Drosophila has implied that a variety of mutations affect ion channels1–4. In one of these studies it was suggested that mutations mapping at the X-linked Shaker locus of D. melanogaster might cause defects in some type of potassium channel in larval motoneurones2. We show here, by voltage-clamp analysis, that mutations at the Shaker locus affect one of the two types of voltage-sensitive potassium channels present in the Drosophila dorsal longitudinal flight muscle (DLM). Initial voltage-clamp studies5,6 revealed that one set of channels carries a fast, transient outward current, similar to the molluscan A current7–9, that is altered by mutations at the Shaker locus. The other set of channels carries a slower activating current, similar to delayed rectification10,11, that is not affected by the Shaker mutation.
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References
Kelly, L. E. Nature 248, 166–168 (1974).
Jan, Y. N., Jan, L. Y. & Dennis, M. J. Proc. R. Soc. B198, 87–108 (1977).
Wu, C.-F., Ganetzky, B., Jan, L. Y., Jan, Y.-N. & Benzer, S. Proc. natn. Acad. Sci. U.S.A. 75, 4047–4051 (1978).
Wu, C.-F. & Ganetzky, B. Nature 286, 814–816 (1980).
Salkoff, L. & Wyman, R. Proc. natn. Acad. Sci. U.S.A. 77, 6216–6220 (1980).
Salkoff, L. & Wyman, R. Science 212, 461–463 (1981).
Hagiwara, S., Kusano, K. & Saito, N. J. Physiol., Lond. 155, 470–489 (1961).
Connor, J. A. & Stevens, C. F. J. Physiol., Lond. 213, 21–30 (1971).
Neher, E. J. gen. Physiol. 58, 36–53 (1971).
Hodgkin, A. L. & Huxley, A. F. J. Physiol., Lond. 116, 473–496 (1952).
Aldrich, R. W., Getting, P. A. & Thompson, S. H. J. Physiol., Lond. 291, 507–530 (1979).
Pasternak, M. & Kaplan, W. D. Drosoph. Inf. Serv. 43, 62 (1968).
Thompson, S. H. J. Physiol., Lond. 265, 465–488 (1977).
Yeh, J. Z., Oxford, G. S., Wu, C. H. & Narahashi, T. Biophys. J. 16, 77–81 (1976).
Schauf, C. L., Colton, C. A., Colton, J. S. & Davis, F. A. J. Pharmac. exp. Ther. 197, 414–425 (1976).
Tanouye, M. A., Ferrus, A. & Fujita, S. C. Proc. natn. Acad. Sci. U.S.A. (in the press).
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Salkoff, L., Wyman, R. Genetic modification of potassium channels in Drosophila Shaker mutants. Nature 293, 228–230 (1981). https://doi.org/10.1038/293228a0
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DOI: https://doi.org/10.1038/293228a0
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