Abstract
The patterns of integration of persisting viral DNA have been examined in 30 to 40 different adenovirus type 2 (Ad2)- and type 12 (Ad12)-transformed hamster cell lines, Ad12-induced hamster and rat tumours and tumour cell lines1–5. These patterns have proved to be quite complex, and so far no evidence has been found for specific sites of virus integration. However, the methods of analysis used so far, are not sufficiently sensitive to decide definitively the issue of specificity. Moreover, specific signals directing the interactions between viral and cellular DNA may reside in DNA sequences distant from the site of junction between the two genomes and may be hidden in hitherto undetected structural peculiarities of DNA sequences. From the DNA of an Ad12-induced hamster tumour line (CLAC3) carrying five copies of the Ad12 genome1, the site of junction between the left terminus of Ad12 DNA and cellular DNA has now been recloned in a λ gtWES·λB′ vector. Determination of the nucleotide sequence at this site of junction reveals that the Ad12 DNA sequence is preserved starting with base pair (bp) 46 of the authentic left end of Ad12 DNA. The first 82 bp of the recloned fragment are not viral and contain scattered, patch-like octa- to undecanucleotide pair homologies to distant sequences within the first 2,722 left terminal base pairs of Ad12 DNA. Close to and encompassing the site of junction a stemmed loop can be constructed based on extensive regions of dyad symmetry. The stability of this loop and its biological function are still uncertain.
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Deuring, R., Winterhoff, U., Tamanoi, F. et al. Site of linkage between adenovirus type 12 and cell DNAs in hamster tumour line CLAC3. Nature 293, 81–84 (1981). https://doi.org/10.1038/293081a0
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DOI: https://doi.org/10.1038/293081a0
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