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Evidence for association of glycosphingolipid with a colchicine-sensitive microtubule-like cytoskeletal structure of cultured cells

Nature volume 293, pages 76–79 (1981)Cite this article

Abstract

Glycosphingolipids are generally considered to be present only on the surface of plasma membranes and to participate in surface-related cellular activity1. However, our previous immunohistochemical studies2 showed that affinity-purified antibody against galactocerebroside (GC) labelled not only the plasma membrane, but also the cytoplasm of epithelial cells in particular areas of hamster kidney, liver and lung. To confirm these findings and to try to obtain more detailed information on the subcellular localization of GC, we reinvestigated this problem in an epithelial cell line derived from monkey kidney. We report here the results indicating that GC is present in the cytoplasm of some cultured cell lines of epithelial origin in close association with colchicine-sensitive microtubule-like sub-cellular structure.

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References

  1. Yamakawa, T. & Nagai, Y. Trends biochem. Sci. 3, 128–131 (1978).

    Article  CAS  Google Scholar 

  2. Sakakibara, K., Iwamori, M., Uchida, T. & Nagai, Y. Experientia 37, 772–774 (1981).

    Article  Google Scholar 

  3. Takaoka, T., Katsuta, H., Endo, M., Sato, K. & Okumura, H. Jap. J. exp. Med. 32, 351–368 (1962).

    CAS  PubMed  Google Scholar 

  4. Ishizuka, I., Tadano, K., Nagata, N., Niimura, Y. & Nagai, Y. Biochim. biophys. Acta 541, 467–482 (1978).

    Article  CAS  PubMed  Google Scholar 

  5. Sakakibara, K. & Tsukada, Y. Gann 71, 679–685 (1980).

    CAS  PubMed  Google Scholar 

  6. Uchida, T. & Nagai, Y. J. Biochem. 87, 1839–1841 (1980).

    Google Scholar 

  7. Sternberger, L. A. in Immunocytochemistry 2nd edn, 354 (Wiley, New York, 1979).

    Google Scholar 

  8. Weber, K., Pollack, R. & Bibring, T. Proc. natn. Acad. Sci. U.S.A. 72, 459–463 (1975).

    Article  ADS  CAS  Google Scholar 

  9. Gordon, W. E., Bushnell, A. & Burridge, K. Cell 13, 249–261 (1978).

    Article  CAS  PubMed  Google Scholar 

  10. Osborn, M. & Weber, K. Cell 12, 561–571 (1977).

    Article  CAS  PubMed  Google Scholar 

  11. Weber, K., Pathke, P. C., Osborn, M. & Franke, W. W. Expl Cell Res. 102, 285–297 (1976).

    Article  CAS  Google Scholar 

  12. Lazarides, E. Nature 283, 249–255 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Hynes, R. O. & Destree, A. T. Cell 13, 151–164 (1978).

    Article  CAS  PubMed  Google Scholar 

  14. Henderson, D. & Weber, K. Expl Cell Res. 134, 301–316 (1979).

    Article  Google Scholar 

  15. Karsenti, E. et al. J. Histochem. Cytochem. 26, 934–947 (1978).

    Article  CAS  PubMed  Google Scholar 

  16. Hakomori, S. Biochim. biophys. Acta 417, 151–163 (1975).

    Google Scholar 

  17. Kido, H., Sanada, Y., Katsunuma, N. & Morris, H. P. Gann 68, 691–696 (1977).

    CAS  PubMed  Google Scholar 

  18. Tadano, K. & Ishizuka, I. Proc. Japan Conf. Lipids 22, 353–357 (1980).

    Google Scholar 

  19. Yahara, S., Singh, I. & Kishimoto, Y. Biochim. biophys. Acta 619, 177–185 (1980).

    Article  CAS  PubMed  Google Scholar 

  20. Jungalwals, F. B. J. Lipid Res. 15, 114–123 (1974).

    Google Scholar 

  21. Herschkowitz, N., McKhann, G. M., Saxena, S. & Shooter, E. M. J. Neurochem. 15, 1181–1188 (1968).

    Article  CAS  PubMed  Google Scholar 

  22. Herschkowitz, N., McKhann, G. M., Saxena, S., Shooter, E. M. & Herndon, R. J. Neurochem. 16, 1049–1057 (1969).

    Article  CAS  PubMed  Google Scholar 

  23. Sonnino, S., Ghidoni, R., Marchesini, S. & Tettamanti, G. J. Neurochem. 33, 117–121 (1979).

    Article  CAS  PubMed  Google Scholar 

  24. Nagai, Y. & Ohsawa, T. Jap. J. exp. Med. 44, 451–464 (1974).

    CAS  PubMed  Google Scholar 

  25. Folch, J., Lees, M. & Sloane-Stanley, G. H. J. biol. Chem. 226, 497–509 (1957).

    CAS  PubMed  Google Scholar 

  26. Saito, T. & Hakomori, S. J. Lipid Res. 12, 257–259 (1971).

    CAS  PubMed  Google Scholar 

  27. Schwarzmann, G. Bochim. biophys. Acta 259, 104–114 (1978).

    Google Scholar 

  28. Momoi, T. & Wiegandt, H. Hoppe-Seyler's Z. physiol. Chem. 361, 1201–1210 (1980).

    Article  CAS  Google Scholar 

  29. Kean, E. L. J. Lipid Res. 7, 449–452 (1966).

    CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Pathobiochemical Cell Research, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Kooko Sakakibara, Takashi Momoi, Tsutomu Uchida & Yoshitaka Nagai

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  1. Kooko Sakakibara
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  2. Takashi Momoi
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  3. Tsutomu Uchida
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  4. Yoshitaka Nagai
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Sakakibara, K., Momoi, T., Uchida, T. et al. Evidence for association of glycosphingolipid with a colchicine-sensitive microtubule-like cytoskeletal structure of cultured cells. Nature 293, 76–79 (1981). https://doi.org/10.1038/293076a0

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