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Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase

Nature volume 291, pages 117–121 (1981)Cite this article

Abstract

Synthesis of the large subunit polypeptide of ribulose bisphosphate carboxylase can be detected in Escherichia coli cells containing the chloroplast genes from maize and wheat. The chloroplast DNA sequences contain a ‘promoter’ that, in Escherichia coli, initiates transcription and translation of the genes. This synthesis of large subunits has been substantially increased using the powerful λ promoter, PL, to drive transcription of the maize gene.

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Authors and Affiliations

  1. Plant Breeding Institute, Trumpington, Cambridge, CB2 2LQ, UK

    Anthony A. Gatenby, Judith A. Castleton & Michael W. Saul

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  1. Anthony A. Gatenby
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  2. Judith A. Castleton
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  3. Michael W. Saul
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Gatenby, A., Castleton, J. & Saul, M. Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase. Nature 291, 117–121 (1981). https://doi.org/10.1038/291117a0

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  • DOI: https://doi.org/10.1038/291117a0

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