Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

N-acetoxy-acetylaminofluorene reacts preferentially with a control region of intracellular SV40 chromosome

Nature volume 291, pages 84–85 (1981)Cite this article

Abstract

Many chemical carcinogens or their metabolites react with DNA1,2; thus it is of interest to determine what effect chromosomal structure has on these reactions. The chromosome of simian virus 40 (SV40) is well suited for such studies; like chromatin of eukaryotic cells, it is organized into nucleosomes. The nucleotide sequence of SV40 is known3,4, together with much about the pattern of viral gene expression and DNA replication5, and the structure of the viral chromosome6–14. We have investigated the binding of the ultimate carcinogen, N-acetoxy-acetylaminofluorene (AAAF), to specific regions of the SV40 chromosome in situ in the intact infected cell. The results, reported here, indicate that a region containing regulatory functions on the intracellular SV40 chromosome has unique structural properties which render it more susceptible to attack by AAAF than the rest of the SV40 genome. The preferential binding of AAAF to regulatory regions of chromatin may have implications for the mechanism of action of this and similar carcinogens.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Sarma, D. S. R., Rajalakshmi, S. & Farber, E. in Cancer Vol. 1 (ed. Becker, F. F.) 235–287 (Plenum, New York, 1975).

    Google Scholar 

  2. Neidle, S. Nature 283, 135 (1980).

    Article  ADS  CAS  Google Scholar 

  3. Fiers, W. et al. Nature 273, 113–120 (1978).

    Article  ADS  CAS  Google Scholar 

  4. Reddy, V. B. et al. Science 200, 494–502 (1978).

    Article  ADS  CAS  Google Scholar 

  5. Tooze, J. (ed.) DNA Tumor Viruses (Cold Spring Harbor Laboratory, New York, 1980).

  6. Bellard, M., Oudet, P., Germond, J.-E. & Chambon, P. Eur. J. Biochem. 70, 543–553 (1976).

    Article  CAS  Google Scholar 

  7. Cremisi, C., Pignatti, P. & Yaniv, M. Biochem. biophys. Res. Commun. 73, 548–554 (1976).

    Article  CAS  Google Scholar 

  8. Ponder, B. A. J. & Crawford, L. V. Cell 11, 35–49 (1977).

    Article  CAS  Google Scholar 

  9. Scott, W. A. & Wigmore, D. J. Cell 15, 1511–1518 (1978).

    Article  CAS  Google Scholar 

  10. Varshavsky, A. J., Sundin, O. & Bohn, M. Cell 16, 453–466 (1979).

    Article  CAS  Google Scholar 

  11. Saragosti, S., Moyne, G. & Yaniv, M. Cell 20, 65–75 (1980).

    Article  CAS  Google Scholar 

  12. Jakobovits, E., Bratosin, S. & Aloni, Y. Nature 285, 263–265 (1980).

    Article  ADS  CAS  Google Scholar 

  13. Beard, P. Cell 15, 955–967 (1978).

    Article  CAS  Google Scholar 

  14. Mathis, D. J., Oudet, P., Wasylyk, B. & Chambon, P. Nucleic Acids Res. 5, 3523–3547 (1978).

    Article  CAS  Google Scholar 

  15. Kriek, E. Biochim. biophys. Acta 355, 177–203 (1974).

    CAS  PubMed  Google Scholar 

  16. Kriek, E., Miller, J. A., Juhl, U. & Miller, E. C. Biochemistry 6, 177–182 (1967).

    Article  CAS  Google Scholar 

  17. Amacher, D. E. & Lieberman, M. W. Biochem. biophys. Res. Commun. 74, 285–290 (1977).

    Article  CAS  Google Scholar 

  18. Kaneko, M. & Cerutti, P. A. Cancer Res. (in the press).

  19. Cech, T. & Pardue, M. Cell 11, 631–640 (1977).

    Article  CAS  Google Scholar 

  20. Wiesehahn, G., Hyde, J. & Hearst, J. Biochemistry 16, 925–932 (1977).

    Article  CAS  Google Scholar 

  21. Jahn, C. & Litman, G. Biochemistry 18, 1442–1449 (1979).

    Article  CAS  Google Scholar 

  22. Metzger, G., Wilhelm, F. & Wilhelm, M. Biochem. biophys. Res. Commun. 75, 703–710 (1977).

    Article  CAS  Google Scholar 

  23. Hirt, B. J. molec. Biol. 26, 365–369 (1967).

    Article  CAS  Google Scholar 

  24. Radloff, F., Bauer, W. & Vinograd, J. Proc. natn. Acad. Sci. U.S.A. 57, 1514–1521 (1967).

    Article  ADS  CAS  Google Scholar 

  25. Champoux, J. J. & McConaughy, B. L. Biochemistry 15, 4638–4642 (1976).

    Article  CAS  Google Scholar 

  26. Sen, A. & Levine, A. J. Nature 249, 343–344 (1974).

    Article  ADS  CAS  Google Scholar 

  27. Young, L. S. & Champoux, J. J. Nucleic Acids Res. 5, 623–635 (1978).

    Article  CAS  Google Scholar 

  28. Levine, A. F., Fink, L. M., Weinstein, I. B. & Grunberger, D. Cancer Res. 34, 319–327 (1974).

    CAS  PubMed  Google Scholar 

  29. Davies, D. R. & Zimmerman, S. Nature 283, 11–12 (1980).

    Article  ADS  CAS  Google Scholar 

  30. Beard, P., Morrow, J. F. & Berg, P. J. Virol. 12, 1303–1313 (1973).

    CAS  PubMed  PubMed Central  Google Scholar 

  31. Mulder, C. & Delius, H. Proc. natn. Acad. Sci. U.S.A. 69, 3215–3219 (1972).

    Article  ADS  CAS  Google Scholar 

  32. Yamasaki, H., Leffler, S. & Weinstein, I. B. Cancer Res. 37, 684–691 (1977).

    CAS  PubMed  Google Scholar 

  33. Dana, K. J. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 69, 3097–3100 (1972).

    Article  ADS  Google Scholar 

  34. Tjian, R. Cell 13, 165–179 (1978).

    Article  CAS  Google Scholar 

  35. Ghosh, P. K., Reddy, V. B., Swinscoe, J., Lebowitz, P. & Weissman, S. J. molec. Biol. 126, 813–846 (1978).

    Article  CAS  Google Scholar 

  36. Handa, H., Kaufman, R. J., Manley, J., Gefter, M. & Sharp, P. A. J. biol. Chem. 256, 478–482 (1981).

    CAS  PubMed  Google Scholar 

  37. Shelton, E. R., Wassarman, P. M. & DePamphilis, M. L. J. molec. Biol. 125, 491–514 (1978).

    Article  CAS  Google Scholar 

  38. Lebowitz, P., Siegel, W. & Sklar, J. J. molec. Biol. 88, 105–123 (1974).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Virology, Swiss Institute for Experimental Cancer Research, 1066, Epalinges, Switzerland

    Peter Beard

  2. Department of Carcinogenesis, Swiss Institute for Experimental Cancer Research, 1066, Epalinges, Switzerland

    Motohisa Kaneko & Peter Cerutti

Authors
  1. Peter Beard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Motohisa Kaneko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Cerutti
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beard, P., Kaneko, M. & Cerutti, P. N-acetoxy-acetylaminofluorene reacts preferentially with a control region of intracellular SV40 chromosome. Nature 291, 84–85 (1981). https://doi.org/10.1038/291084a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/291084a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing