Abstract
It is now well established that in the mouse, helper T cells and killer T cells are two distinct thymus-derived lymphocyte sub-populations, differing from each other in Lyt phenotype and H–2 restriction, among other parameters. Helper T cells are Lyt-1+ and their action in immune responses involves restriction at the H–2I region of the major histocompatibility complex (MHC)1,2. Killer cells, on the other hand, are Lyt-23+ and their activity is restricted by H–2K/D (refs 1, 3). In most instances, T cells mediating delayed-type hypersensitivity (DTH) responses share the Lyt phenotype and H–2 restriction of the helper T cell4–7. This raises the question of whether or not helper activity and DTH can be mediated by the same activated T cell. Arguments for both views have been reported8–12. We analysed this question using clones of specific helper T cells, which were obtained by long-term culture in vitro of in vivo primed T cells, followed by single-cell cloning13–15. Here we show that these clones of helper T cells mediate antigen-specific and fully H–2-restricted DTH. The restricting element lies to the left of the I-B region in genetic maps of the mouse MHC.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cantor, H. & Boyse, E. A. J. exp. Med. 141, 1376–1389 (1975).
Sprent, J. Immun. Rev., 42, 108–148 (1978).
Bevan, M. J. J. exp. Med. 142, 1349–1364 (1975).
Miller, J. F. A. P., Vadas, M. A., Whitelaw, A. & Gamble, J. Proc. natn. Acad. Sci. U.S.A. 72, 5095–5098 (1975).
Vadas, M. A. et al. J. exp. Med. 144, 10–19 (1976).
Vadas, M. A., Miller, J. F. A. P., Whitelaw, A. M. & Gamble, J. R. Immunogenetics 144, 137–153 (1977).
Smith, F. & Miller, J. F. A. P. Int. Archs Allergy appl. Immun. 58, 295–301 (1979).
Parish, C. R. in Progress in Immunology Vol. 2 (eds Brent, L. & Holborow, J.) 39–44 (North-Holland, Amsterdam, 1974).
Lubet, M. T. & Kettman, J. R. J. Immun. 123, 426–433 (1979).
Ramshaw, J. A. & Eidenger, D. Cell. Immun. 42, 42–47 (1979).
Lagrange, P. H. & Mackaness, G. B. J. exp. Med. 148, 235–245 (1978).
Kerckhaert, J. A. M. & Hofhuis, F. M. A. Annls Inst. Pasteur, Paris 126C, 371–382 (1975).
Schreier, M. H. & Tees, R. Int. Archs Allergy appl. Immun. 61, 227–237 (1980).
Schreier, M. H., Iscove, N. N., Tees, R., Aarden, L. & von Boehmer, H. Immun. Rev. 51, 315–336 (1980).
Tees, R. & Schreier, M. H. Nature 283, 780–781 (1980).
Brent, L., Brown, J. B. & Medawar, P. B. Proc. R. Soc. B156, 187–209 (1962).
Lubet, M. T. & Kettman, J. R. Cell. Immun. 45, 151–161 (1979).
van der Kwast, T. H. J. Immunogenet. 7, 315–324 (1980).
Zinkernagel, R. M. J. exp. Med. 144, 776–787 (1976).
Elliott, B. E., Haskill, J. S. & Axelrad, M. A. J. exp. Med. 141, 584–599 (1975).
Schreier, M. H., Andersson, J., Lernhardt, W. & Melchers, F. J. exp. Med. 151, 194–203 (1980).
Melchers, F., Andersson, J., Lernhardt, W. & Schreier, M. H. Eur. J. Immun. 10, 679–685 (1980).
Schreier, M. H. & Iscove, N. N. Nature 287, 228–230 (1980).
Phillips, S. M., Carpenter, C. B. & Merril, J. P. Cell. Immun. 5, 249–263 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bianchi, A., Hooijkaas, H., Benner, R. et al. Clones of helper T cells mediate antigen-specific, H–2-restricted DTH. Nature 290, 62–63 (1981). https://doi.org/10.1038/290062a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/290062a0
This article is cited by
-
Stimulation of prostaglandin formation by an antigen- and Ia-restricted T-cell-macrophage interaction
Agents and Actions (1986)
-
Suppression of DTH mediated by cloned helper T cells with ciclosporin and dexamethasone
Agents and Actions (1986)
-
Joint inflammations and flare-up reactions in mice induced by a helper T cell clone
Agents and Actions (1986)
-
Late asthmatic responses: Inquiry into mechanisms and significance
Clinical Reviews in Allergy (1985)
-
Restricted recognition of H-2 subregion coded alloantigens in delayed-type hypersensitivity
Immunogenetics (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
