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Intervening sequences in a plant gene—comparison of the partial sequence of cDNA and genomic DNA of French bean phaseolin

Nature volume 289, pages 37–41 (1981)Cite this article

Abstract

A plant gene coding for the major storage protein (phaseolin, G1-globulin) of the French bean was isolated from a genomic library constructed in the phage vector Charon 24A. Comparison of the nucleotide sequence of part of the gene with that of the cloned messenger RNA (cDNA) revealed the presence of three intervening sequences, all beginning with GTand ending with AG. The 5′; and 3′ boundaries of intervening sequences TVS-A (88 base pairs) and IVS-B (124 base pairs) are similar to those described for animal and viral genes, but the 3′ boundary of IVS-C (129 base pairs) shows some differences. A sequence of 185 amino acids deduced from the cloned DMAs represents about 40% of a phaseolin polypeptide.

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Author notes

  1. S. M. Sun

    Present address: Atlantic Richfield Co., 6905 Sierra Court, PO Box 2600, Dublin, California, 94566

  2. J. L. Slightom

    Present address: Departments of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois, 62901

Authors and Affiliations

  1. Departments of Horticulture and Genetics, University of Wisconsin, Madison, Wisconsin, 53706

    J. L. Slightom

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  3. T. C. Hall
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Sun, S., Slightom, J. & Hall, T. Intervening sequences in a plant gene—comparison of the partial sequence of cDNA and genomic DNA of French bean phaseolin. Nature 289, 37–41 (1981). https://doi.org/10.1038/289037a0

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