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Recognition sequence of bacteriophage ΦX174 gene A protein — an initiator of DNA replication

Nature volume 288, pages 561–566 (1980)Cite this article

Abstract

Gene A protein, the initiator protein of bacteriophage ΦX174 DNA replication, cleaves synthetic single-stranded oligodeoxyribonucleotides at the same site as the corresponding sequence at the ΦX origin. The results identify the recognition sequence within the decamer CAACTTGATA which is cleaved next to the G residue. Further requirements for cleavage of double-stranded DNA by the gene A protein are supercoiling and an A + T-rich domain adjacent to the recognition sequence.

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Authors and Affiliations

  1. Institute of Molecular Biology, State University of Utrecht, Utrecht, The Netherlands

    A. D. M. van Mansfeld, S. A. Langeveld, P. D. Baas & H. S. Jansz

  2. Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands

    A. D. M. van Mansfeld, P. D. Baas & H. S. Jansz

  3. Department of Organic Chemistry, State University of Leiden, Leiden, The Netherlands

    G. A. van der Marel, G. H. Veeneman & J. H. van Boom

Authors
  1. A. D. M. van Mansfeld
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  2. S. A. Langeveld
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  3. P. D. Baas
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  4. H. S. Jansz
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  5. G. A. van der Marel
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  6. G. H. Veeneman
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  7. J. H. van Boom
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van Mansfeld, A., Langeveld, S., Baas, P. et al. Recognition sequence of bacteriophage ΦX174 gene A protein — an initiator of DNA replication. Nature 288, 561–566 (1980). https://doi.org/10.1038/288561a0

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