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The λ phage att site: functional limits and interaction with Int protein

Nature volume 285, pages 85–91 (1980)Cite this article

Abstract

Site-specific integrative recombination of bacteriophage λ involves unequal partners. The minimal phage att site is composed of approximately 240-base pairs and four distinct binding sites for Int protein, at least three of which are crucial for function. This ‘donor site’ recombines efficiently with a smalle ‘recipient site’ that lacks the extensive interactions with Int protein.

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References

  1. Gottesman, M. E. & Weisberg, R. A. in The Bacteriophage Lambda (ed. Hershey, A.D.) 113–138 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  2. Nash, H. A. Curr. Topics Microbiol. Immun. 78, 171–199 (1978).

    MathSciNet  Google Scholar 

  3. Miller, H. I., Kikuchi, A., Nash, H. A., Weisberg, R. A. & Friedman, D. I. Cold Spring Harb. Symp. quant. Biol. 43, 1121–1126 (1979).

    Article  CAS  Google Scholar 

  4. Hoess, R., Foeller, C., Bidwell, K. & Landy, A. Proc. natn. Acad. Sci. U.S.A. (in the press).

  5. Gottesman, S. & Abremski, K. J. molec. Biol. 138 (in the press).

  6. Kotewicz, M., Chung, S., Takeda, Y. & Echols, H. Proc. natn. Acad. Sci. U.S.A. 74, 1511–1515 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Kikuchi, Y. & Nash, H. A. J. biol. Chem. 253, 7149–7157 (1978).

    CAS  PubMed  Google Scholar 

  8. Kikuchi, Y. & Nash, H. A. Cold Spring Harb. Symp. quant. Biol. 43, 1099–1109 (1979).

    Article  CAS  Google Scholar 

  9. Ross, W., Landy, A., Kikuchi, Y. & Nash, H. A. Cell 18, 297–307 (1979).

    Article  CAS  Google Scholar 

  10. Kikuchi, A. & Nash, H. A. Proc. natn. Acad. Sci. U.S.A. 76, 3760–3764 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Mizuuchi, K. & Mizuuchi, M. Cold Spring Harb. Symp. quant. Biol. 43, 1111–1114 (1979).

    Article  CAS  Google Scholar 

  12. Mizuuchi, K., Gellert, M. & Nash, H. A. J. molec. Biol. 121, 375–392 (1978).

    Article  CAS  Google Scholar 

  13. Landy, A. & Ross, W. Science 197, 1147–1160 (1977).

    Article  ADS  CAS  Google Scholar 

  14. Guerrini, F. J. molec. Biol. 46, 523–542 (1969).

    Article  CAS  Google Scholar 

  15. Echols, H. J. molec. Biol. 47, 575–583 (1970).

    Article  CAS  Google Scholar 

  16. Guarneros, G. & Echols, H. Virology 52, 30–38 (1973).

    Article  CAS  Google Scholar 

  17. Galas, D. J. & Schmitz, A. Nucleic Acids Res. 5, 3157–3170 (1978).

    Article  CAS  Google Scholar 

  18. Davies, R. W., Schreier, P. H., Kotewicz, M. L. & Echols, H. Nucleic Acids Res. 7, 2255–2273 (1979).

    Article  CAS  Google Scholar 

  19. Pollock, T. J. & Abremski, K. J. molec. Biol. 131, 651–654 (1979).

    Article  CAS  Google Scholar 

  20. Nash, H. A. Proc. natn. Acad. Sci. U.S.A. 72, 1072–1076 (1975).

    Article  ADS  CAS  Google Scholar 

  21. Gottesman, S. & Gottesman, M. E. J. molec. Biol. 91, 489–499 (1975).

    Article  CAS  Google Scholar 

  22. Mizuuchi, M. & Mizuuchi, K. Proc. natn. Acad. Sci. U.S.A. in the press.

  23. Finch, J. T. et al. Nature 269, 29–36 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Noll, M. Nucleic Acids Res. 1, 1573–1578 (1974).

    Article  CAS  Google Scholar 

  25. Lutter, L. C. Nucleic Acids Res. 6, 41–56 (1979).

    Article  ADS  CAS  Google Scholar 

  26. Liu, L. F. & Wang, J. C. Cell 15, 979–984 (1978).

    Article  CAS  Google Scholar 

  27. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  28. D'Andrea, A. D. & Haseltine, W. Proc. natn. Acad. Sci. U.S.A. 75, 3608–3612 (1978).

    Article  ADS  CAS  Google Scholar 

  29. Hatayama, T., Goldberg, I., Takeshita, M. & Grollman, A. Proc. natn. Acad. Sci. U.S.A. 75, 3603–3607 (1978).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Section of Microbiology and Molecular Biology, Division of Biology and Medicine, Brown University, Providence, Rhode Island, 02912

    Pei-Ling Hsu, Wilma Ross & Arthur Landy

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  1. Pei-Ling Hsu
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  2. Wilma Ross
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  3. Arthur Landy
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Hsu, PL., Ross, W. & Landy, A. The λ phage att site: functional limits and interaction with Int protein. Nature 285, 85–91 (1980). https://doi.org/10.1038/285085a0

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