Abstract
The interaction between protein and water is of fundamental importance for processes ranging from protein folding and enzymatic activity1 to anhydrobiosis2. In this letter we bring together results from diverse types of measurements to give a unified picture of the hydration process for lysozyme. The data come principally from experiments with protein films and powders. The principal aim is to examine the relationship between the sites of water interaction, the extent of coverage, and the enzymatic activity, thus providing a better understanding of the relationship between water and enzyme dynamics3.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kuntz, I. D. & Kauzmann, W. Adv. Protein Chem. 28, 239–345 (1974).
Clegg, J. S. in Cell-Associated Water (eds Drost-Hansen, W. & Clegg, J. S.) 363–413 (Academic, New York, 1979).
Careri, G., Fasella, P. and Gratton, E. A. Rev. Biophys. Bioengng 8, 69–97 (1979).
Yang, P.-H. & Rupley, J. A. Biochemistry 18, 2654–2661 (1979).
Rupley, J. A., Yang, P.-H. & Tollin, G. in Water in Polymers (ed. Rowland, S. P.) (American Chemical Society, in the press).
Careri, G., Giansanti, A. & Gratton, E. Biopolymers 18, 1187–1203 (1979).
Careri, G., DeAngelis, L., Gratton, E. & Messana, C. Phys. Lett. 60A, 490–491 (1977).
Lee, B. & Richards, F. M. J. molec. Biol. 55, 379–400 (1971).
Shrake, A. & Rupley, J. A. J. molec. Biol. 79, 351–371 (1973).
Doyle, B. B., Bendit, E. G. & Blout, E. R. Biopolymers 14, 937–957 (1975).
Hill, T. L. J. chem. Phys. 17, 762–771 (1949).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Careri, G., Gratton, E., Yang, PH. et al. Correlation of IR spectroscopic, heat capacity, diamagnetic susceptibility and enzymatic measurements on lysozyme powder. Nature 284, 572–573 (1980). https://doi.org/10.1038/284572a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/284572a0
This article is cited by
-
Revisiting macromolecular hydration with HullRadSAS
European Biophysics Journal (2023)
-
Evaluation of protein secondary structure from FTIR spectra improved after partial deuteration
European Biophysics Journal (2021)
-
Photosystem II: Structure and mechanism of the water:plastoquinone oxidoreductase
Photosynthesis Research (2007)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.