Abstract
Contraction of vertebrate skeletal muscle is caused by calcium ions released from the sarcoplasmic reticulum (see refs 1, 2 for reviews). The ensuing transient change in the intracellular level of ionised calcium has been monitored using various Ca2+ indicators, such as murexide,3 aequorin4,5, and arsenazo III6,7. So far, most of what is known about these calcium transient derives from experiments on barnacle or frog muscle fibres, and it is desirable to extend such studies to mammalian muscle. We report here that the photoprotein aequorin8,9 can be used to monitor calcium transients in rat and human muscles, and that the transients decay more quickly in fast contracting muscle fibres.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ebashi, S. A. Rev. Physiol. 38, 293–313 (1976).
Endo, M. Physiol. Rev. 57, 71–108 (1977).
Jöbsis, F. F. & O'Connor, M. J. Biochem. biophys. Res. Commun. 25, 246–252 (1966).
Ashley, C. C. & Ridgway, E. B. J. Physiol., Lond. 209, 105–130 (1970).
Blinks, J. R., Rüdel, R. & Taylor, S. R. J. Physiol., Lond. 277, 291–323 (1978).
Miledi, R., Parker, I. & Schalow, G. Proc. R. Soc. B198, 201–210 (1977).
Miledi, R., Parker, I. & Schalow, G. Nature 268, 750–752 (1977).
Shimomura, O., Johonson, R. H. & Saiga, Y. J. cell. comp. Physiol. 59, 223–239 (1962).
Blinks, J. R., Prendergast, F. G. & Allen, D. G. Pharmac. Rev. 28, 1–93 (1976).
Miledi, R. & Slater, C. R. Proc. R. Soc. B169, 289–306 (1968).
Kusano, K., Miledi, R. & Stinnakre, J. Proc. R. Soc. B189, 39–47 (1975).
Katz, B. & Miledi, R. J. Physiol., Lond. 189, 535–544 (1967).
Close, R. J. Physiol., Lond. 193, 45–55 (1967).
Schiaffino, S., Hanzlikova, V. & Pierobon, S. J. Cell Biol. 47, 107–119 (1970).
Luff, A. R. & Atwood, H. L. J. Cell Biol. 51, 369–383, (1971).
Sréter, F. A. & Gergely, J. Biochem. biophys. Res. Commun. 16, 438–448 (1964).
Harigaya, S., Ogawa, Y. & Sugita, H. J. Biochem. 63, 324–331 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eusebi, F., Miledi, R. & Takahashi, T. Calcium transients in mammalian muscles. Nature 284, 560–561 (1980). https://doi.org/10.1038/284560a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/284560a0
This article is cited by
-
Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers
Nature Communications (2020)
-
Effect of 5-day vitamin E supplementation on muscle injury after downhill running in rats
European Journal of Applied Physiology (2011)
-
Different fibre populations distinguished by their calcium transient characteristics in enzymatically dissociated murine flexor digitorum brevis and soleus muscles
Journal of Muscle Research and Cell Motility (2009)
-
RyR isoforms and fibre type-specific expression of proteins controlling intracellular calcium concentration in skeletal muscles
Journal of Muscle Research and Cell Motility (2006)
-
Characterization study of the ryanodine receptor and of calsequestrin isoforms of mammalian skeletal muscles in relation to fibre types
Journal of Muscle Research and Cell Motility (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
