Abstract
An approach is described for determining secondary structure in large RNA molecules, in which families of short interacting RNA fragments are isolated and identified as such by two-dimensional gel electrophoresis. Data covering over half of the 16S ribosomal RNA from Escherichia coli are presented, including two long-range interactions.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Noller, H. F. Biochemistry 13, 4694–4703 (1974).
Chapman, N. M. & Noller, H. F. J. molec. Biol. 109, 131–149 (1977).
Hogan, J. J. & Noller, H. F. Biochemistry 17, 587–593 (1978).
Uhlenbeck, O. C., Baller, J. & Doty, P. Nature 225, 508–510 (1970).
Wrede, P., Pongs, O. & Erdmann, V. A. J. molec. Biol. 120, 83–96 (1978).
Ehresmann, C. et al. Nucleic Acids Res. 2, 265–278 (1975).
Fiers, W. et al. Nature 260, 500–507 (1976).
Gross, H. J. et al. Nature 273, 203–208 (1978).
Studnicka, G. M., Rahn, G. M., Cummings, I. W. & Salser, W. A. Nucleic Acids Res. 5, 3365–3387 (1978).
Wellauer, P. K. & Dawid, I. B. J. molec. Biol. 89, 379–395 (1974).
Mackie, G. A. & Zimmermann, R. A. J. biol. Chem. 250, 4100–4112 (1975).
Ungewickell, E., Ehresmann, C., Stiegler, P. & Garrett, R. A. Nucleic Acids Res. 2, 1867–1893 (1975).
Rinke, J., Yuki, A. & Brimacombe, R. Eur. J. Biochem. 64, 77–89 (1976).
Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. Proc. natn. Acad. Sci. U.S.A. 75, 4801–4805 (1978).
Carbon, P., Ehresmann, C., Ehresmann, B. & Ebel, J. P. FEBS Lett. 94, 152–156 (1978).
Vogt, V. M. Eur. J. Biochem. 33, 192–200 (1973).
Vigne, R. & Jordan, B. R. Biochimie 53, 981–986 (1971).
Möller, K. & Brimacombe, R. Molec. gen. Genet. 141, 343–355 (1975).
Sanger, F., Brownlee, G. G. & Barrell, B. G. J. molec. Biol. 13, 373–398 (1965).
Hochkeppel, H. K., Spicer, E. & Craven, G. R. J. molec. Biol. 101, 155–170 (1976).
Harada, F. & Dahlberg, J. E. Nucleic Acids Res. 2, 865–871 (1975).
Araco, A., Belli, M. & Onori, G. Nucleic Acids Res. 2, 373–381 (1975).
Müller, R., Garrett, R. A. & Noller, H. F. J. biol. Chem. 254, 3873–3878 (1979).
Ehresmann, C., Stiegler, P., Fellner, P. & Ebel, J. P. Biochimie 57, 711–748 (1975).
Yuki, A. & Brimacombe, R. Eur. J. Biochem. 56, 23–24 (1975).
Rinke, J., Ross, A. & Brimacombe, R. Eur. J. Biochem. 76, 189–196 (1977).
Morgan, J. & Brimacombe, R. Eur. J. Biochem. 29, 542–552 (1972).
Maxam, A. M. & Gilbert, G. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Zwieb, C. & Brimacombe, R. Nucleic Acids Res. 5, 1189–1206 (1978).
Volckaert, G. & Fiers, W. Analyt. Biochem. 83, 228–239 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ross, A., Brimacombe, R. Experimental determination of interacting sequences in ribosomal RNA. Nature 281, 271–276 (1979). https://doi.org/10.1038/281271a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/281271a0
This article is cited by
-
Nuclease S1 analysis of eubacterial 5S rRNA secondary structure
Journal of Molecular Evolution (1985)
-
The primary structure of 16S rDNA from Zea mays chloroplast is homologous to E. coli 16S rRNA
Nature (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.