Abstract
THE identification of a new chicken α-globin gene is reported here. Nucleotide sequence analysis of clone pHb1003 shows it to code for an α-subunit with an amino acid sequence different from those normally observed in the adult (the αA and αD). Characterisation of a second clone, pHb1010, carrying the same novel sequence, suggests that this mRNA sequence was present in substantial quantity after very young (2–3-week-old) chickens were treated with phenylhydrazine. The results are therefore similar to those reported previously for sheep and goats, where treatment with phenylhydrazine results in the turn-on of a new stress globin gene. As the ease of obtaining nucleated erythrocytes from avian systems facilitates many types of biochemical studies, we believe that this finding may provide an especially useful system in which to study the molecular events involved in globin gene switching.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Padayatty, J. et al. Nucleic Acids Res. (submitted).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 570 (1977).
Matsuda, G., Takei, H., Wu, K. & Shiozawa, T. Int. J. Protein. Res. 111, 173 (1971).
Dayhoff, M. Atlas of Protein Sequence and Structure, Vol. 5 (National Biomedical Research Foundations, Silver Spring, 1972).
Takei, H., Ota, Y., Wu, K., Kiyohara, T. & Matsuda, G. J. Biochem. 77, 1345 (1975).
Schalekamp, M., van Goor, D. & Sungerland, R. J. Embryol. exp. Morph. 28, 681 (1972).
Moss, B. & Thompson, E. O. P. Aust. J. biol. Sci. 22, 1455 (1969).
Brown, J. & Ingram, V. J. biol. Chem. 249, 3960 (1974).
Kendrew, J. et al. Nature 185, 422 (1960).
Perutz, M. Br. med. Bull. 32, 195 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CUMMINGS, I., LIU, A. & SALSER, W. Identification of a new chicken α-globin structural gene by complementary DNA cloning. Nature 276, 418–419 (1978). https://doi.org/10.1038/276418a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/276418a0
This article is cited by
-
No evidence for ‘stress’ α-globin genes in chicken
Nature (1982)
-
Restriction patterns of adult chicken globin genes at early and late stages of embryonic development
Molecular Biology Reports (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
