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Mode of coupling between hormone receptors and adenylate cyclase elucidated by modulation of membrane fluidity

Nature volume 276, pages 394–396 (1978)Cite this article

Abstract

THE hormone- and neurotransmitter-dependent adenylate cyclases represent transmembrane regulatory systems in which the receptor unit faces the outside of the cell and the cyclase catalytic unit faces the inside. When the hormone or neurotransmitter binds to the receptor, the catalytic unit of the cyclase is activated and produces cyclic AMP at the inner surface of the membrane1. It has recently become clear that some hormone receptors are permanently coupled to adenylate cyclase whereas others are not. We demonstrate here that the effects of modulating membrane fluidity on the rate of adenlyate cyclase activation can be used to distinguish between the two modes of coupling.

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Authors and Affiliations

  1. Department of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel

    GILAD RIMON, EMANUAL HANSKI, SERGEI BRAUN & ALEXANDER LEVITZKI

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  1. GILAD RIMON
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  2. EMANUAL HANSKI
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  3. SERGEI BRAUN
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  4. ALEXANDER LEVITZKI
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RIMON, G., HANSKI, E., BRAUN, S. et al. Mode of coupling between hormone receptors and adenylate cyclase elucidated by modulation of membrane fluidity. Nature 276, 394–396 (1978). https://doi.org/10.1038/276394a0

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