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Specific poly-A-binding protein of 76,000 molecular weight in polyribosomes is not present on poly A of free cytoplasmic mRNP

Nature volume 270, pages 189–191 (1977)Cite this article

Abstract

CYTOPLASM of animal cells contains mRNA associated with ribosomes (in polysomes) and mRNA free of ribosome attachment. Both classes of mRNA occur as ribonucleoprotein (mRNP) complexes1–3. The role of the untranslated pool of free mRNA (referred to as informosomes3) is uncertain. They represent the form in which mRNA exists in transit from the nucleus to the cytoplasm awaiting association with ribosomes3, and in addition the pool of free cytoplasmic mRNA contains inactive (or inactivated) and storage forms of mRNA4. It has been established that most of the polysomal mRNP complexes contain predominantly two proteins with approximate molecular weights (MW) of 49,000–52,000 and 73,000–78,000 respectively5–9. It is generally accepted that the larger of these proteins is attached to the 3′ poly A tail of mRNA10–12. We present evidence here that the poly A segments of non-polysomal mRNA of Ehrlich ascites tumour cells and of rabbit reticulocytes—in contrast to the polysomal poly A segments10–13—do not contain the 76,000 MW protein.

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References

  1. Williamson, R. FEBS Lett. 37, 1–6 (1973).

    Article  CAS  PubMed  Google Scholar 

  2. Greenberg, J. R. J. Cell Biol. 64 269–288 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Spirin, A. S. Eur. J. Biochem. 10, 20–35 (1969).

    Article  CAS  PubMed  Google Scholar 

  4. Scherrer, K. Cold Spring Harb. Symp., Symp. quant. Biol. 35, 539–554 (1970).

    Article  CAS  Google Scholar 

  5. Bryan, R. N. & Hayashi, M. Nature new Biol. 244, 271–274 (1973).

    Article  CAS  PubMed  Google Scholar 

  6. Greenberg, J. R. J. molec. Biol. 108, 403–416 (1977).

    Article  Google Scholar 

  7. Lindberg, U. & Sundquist, B. J. molec. Biol. 86, 451–468 (1974).

    Article  CAS  PubMed  Google Scholar 

  8. Barrieux, A., Ingraham, H. A., Nystul, S. & Rosenfeld, M. G. Biochemistry 15, 3523–3528 (1976).

    Article  CAS  PubMed  Google Scholar 

  9. Liautard, J. P., Setyono, B., Spindler, E. & Köhler, K. Biochim. biophys. Acta 425, 373–383 (1976).

    Article  CAS  PubMed  Google Scholar 

  10. Blobel, G. Proc. natn. Acad. Sci. U.S.A. 70, 924–928 (1973).

    Article  ADS  CAS  Google Scholar 

  11. Schwartz, H. & Darnell, J. E. J. molec. Biol. 104, 833–857 (1976).

    Article  CAS  PubMed  Google Scholar 

  12. Kish, V. M. & Pederson, T. J. biol. Chem. 251, 5888–5894 (1976).

    CAS  PubMed  Google Scholar 

  13. Janssen, D. B., Counotte-Potman, A. D. & Van Venrooij, W. J. Molec. Biol. Rep. 3, 87–95 (1976).

    Article  CAS  Google Scholar 

  14. Van Venrooij, W. J. & Janssen, A. P. M. Eur. J. Biochem. 69, 55–60 (1976).

    Article  CAS  Google Scholar 

  15. Laemmli, U. K. Nature new Biol. 227, 680–686 (1970).

    Article  ADS  CAS  Google Scholar 

  16. Van Venrooij, W. J., Jansen, R. T. P. & Janssen, D. B. Biochem. Soc. Trans. 5, 662–663 (1977).

    Article  CAS  PubMed  Google Scholar 

  17. Marbaix, G., Huez, G., Nokin, P. & Cleuter, Y. FEBS Lett. 66, 269–273 (1976).

    Article  CAS  PubMed  Google Scholar 

  18. Sheiness, D. & Darnell, J. E. Nature new Biol. 241, 265–268 (1973).

    Article  CAS  PubMed  Google Scholar 

  19. Nokin, P., Huez, G., Marbaix, G., Burny, A. & Chantrenne, H. Eur. J. Biochem. 62, 509–517 (1976).

    Article  CAS  PubMed  Google Scholar 

  20. Merkel, C. G., Gordon Wood, T. & Lingrel, J. B. J. biol. Chem. 251, 5512–5515 (1976).

    CAS  PubMed  Google Scholar 

  21. Bonanou-Tzedaki, S. A., Pragnell, I. B. & Arnstein, H. R. V. FEBS Lett. 26, 77–82 (1972).

    Article  CAS  PubMed  Google Scholar 

  22. Jacobs-Lorena, M. & Baglioni, C. Eur. J. Biochem. 35, 559–565 (1973).

    Article  CAS  PubMed  Google Scholar 

  23. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  24. Slegers, H. & Kondo, M. Nucleic Acids Res. 4, 625–639 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Hunt, L. Virology 70, 484–492 (1976).

    Article  CAS  PubMed  Google Scholar 

  26. Pelham, H. R. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).

    Article  CAS  PubMed  Google Scholar 

  27. Salden, M. H. L., Gielkens, A. L. J. & Bloemendal, H. Biochim. biophys. Acta 425, 208–219 (1976).

    Article  CAS  PubMed  Google Scholar 

  28. Weber, K. & Osborn, M. J. biol. Chem. 244, 4406–4412 (1969).

    CAS  PubMed  Google Scholar 

  29. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    Article  CAS  PubMed  Google Scholar 

  30. Civelli, O., Vincent, A., Buri, J. F. & Scherrer, K. FEBS Lett. 72, 71–76 (1976).

    Article  CAS  PubMed  Google Scholar 

  31. Vincent, A., Civelli, O., Buri, J. F. & Scherrer, K. FEBS Lett. 77, 281–286 (1977).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Nijmegen, Geert Grooteplein Noord 21, Nijmegen, The Netherlands

    W. J. VAN VENROOIJ, C. A. G. VAN EEKELEN, R. T. P. JANSEN & J. M. G. PRINCEN

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  1. W. J. VAN VENROOIJ
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  2. C. A. G. VAN EEKELEN
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  3. R. T. P. JANSEN
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  4. J. M. G. PRINCEN
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VAN VENROOIJ, W., VAN EEKELEN, C., JANSEN, R. et al. Specific poly-A-binding protein of 76,000 molecular weight in polyribosomes is not present on poly A of free cytoplasmic mRNP. Nature 270, 189–191 (1977). https://doi.org/10.1038/270189a0

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