Abstract
INDUCTION of neoplastic transformation in vitro by X rays1–5 and neutrons4 has been reported. Using short term cultures of hamster embryo cells we found previously1–4 that transformation by X rays can be detected with doses as low as 1 rad. The rate of transformation increases with dose reaching a peak of 1% between 150 and 300 rad. This frequency of neoplastic transformation in vitro is much higher than the frequency of radiation-induced tumours observed after exposing animals to similar doses of radiation6. We report here that malignant transformed cells can be obtained from embryos irradiated in utero and assayed in vitro and that the frequency of transformation is at least tenfold lower than that obtained when the irradiations are performed in vitro, and thus closer to the incidence in animals.
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BOREK, C., PAIN, C. & MASON, H. Neoplastic transformation of hamster embryo cells irradiated in utero and assayed in vitro. Nature 266, 452–454 (1977). https://doi.org/10.1038/266452a0
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DOI: https://doi.org/10.1038/266452a0
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