Abstract
Reovirus mRNAs with 5′-terminal m7GpppGm or GpppG are more stable than mRNA containing unblocked ppG 5′-ends when injected into Xenopus laevis oocytes or incubated in cell-free protein synthesising extracts of wheat germ and mouse L cells. The greater stability of mRNA with blocked 5′ termini is not dependent upon translation but seems to result from protection against 5′-exonucleolytic degradation.
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Shatkin, A. J. Cell 9, 645–653 (1976).
Both, G. W., Banerjee, A. K. & Shatkin, A. J. Proc. natn. Acad. Sci. U.S.A. 72, 1189–1193 (1975).
Shih, D. S., Dasgupta, R. & Kaesberg, P. J. Virol. 19, 637–642 (1976).
Muthukrishnan, S., Both, G. W., Furuichi, Y. & Shatkin, A. J. Nature 255, 33–37 (1975).
Muthukrishnan, S. et al. J. biol. Chem. 250, 9336–9341 (1975).
Anderson, C. W., Atkins, J. F. & Dunn, J. J. Proc. natn. Acad. Sci. U.S.A. 73, 2752–2756 (1976).
Toneguzzo, F. & Ghosh, H. P. J. Virol. 17, 477–491 (1976).
Both, G. W., Furuichi, Y., Muthukrishnan, S. & Shatkin, A. J. Cell 6, 185–195 (1975).
Kozak, M. & Shatkin, A. J. J. biol. Chem. 251, 4259–4266 (1976).
Allende, C. C., Allende, J. E. & Firtel, R. A. Cell 2, 189–196 (1974).
Furuichi, Y. & Shatkin, A. J. Proc. natn. Acad. Sci. U.S.A. 73, 3448–3452 (1976).
Furuichi, Y., Muthukrishnan, S., Tomasz, J. & Shatkin, A. J. J. biol. Chem. 251, 5043–5053 (1976).
Nuss, D. L., Furuichi, Y., Koch, G. & Shatkin, A. J. Cell 6, 21–27 (1975).
Shinshi, H., Miwa, M., Sugimura, T., Shimotohno, K. & Miura, K. FEBS Lett. 65, 254–257 (1976).
Ouellete, A. J., Reed, S. L. & Malt, R. A. Proc. natn. Acad. Sci. U.S.A. 73, 2609–2613 (1976).
Perry, R. P. & Kelley, D. E. Cell 8, 433–442 (1976).
Gurdon, J. B., Lane, C. D., Woodland, H. R. & Marbaix, G. Nature 233, 177–182 (1971).
Lee, Y. F., Nomoto, A., Detjen, B. M. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 59–63 (1977).
Furuichi, Y., Shatkin, A. J., Stavnezer, E. & Bishop, J. M. Nature 257, 618–620 (1975).
Pawson, T., Martin, G. S. & Smith, A. E. J. Virol. 19, 950–967 (1976).
Muthukrishnan, S., Morgan, M., Banerjee, A. K. & Shatkin, A. J. Biochemistry 15, 5761–5768 (1976).
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Furuichi, Y., LaFiandra, A. & Shatkin, A. 5′-Terminal structure and mRNA stability. Nature 266, 235–239 (1977). https://doi.org/10.1038/266235a0
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DOI: https://doi.org/10.1038/266235a0
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