Abstract
MUSCARINIC receptors have been characterised extensively in intact smooth muscle1–3 and in subcellular fractions from smooth muscle2,4–6 and brain7–10 by reversible binding of labelled atropine1,9,10, propylbenzilylcholine9,10, quinuclidinylbenzilate (QNB) (refs 6, 8) and benzetimide4,5, or by alkylation with benzilylcholine2 or propylbenzilylcholine mustard3,7,9–11. Several attempts to solubilise muscarinic receptors2,4,12 have failed due to the inhibition of receptor binding by the detergent used for solubilisation2,4. We report here the solubilisation with 2M sodium chloride of an atropine-binding material from the ox cerebral cortex, which has properties similar to those found for the muscarinic receptor in subcellular preparations.
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CARSON, S., GODWIN, S., MASSOULIE, J. et al. Solubilisation of atropine-binding material from brain. Nature 266, 176–178 (1977). https://doi.org/10.1038/266176a0
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DOI: https://doi.org/10.1038/266176a0
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