Abstract
Digestion of mouse liver nuclei with DNase II leads to a novel cleavage pattern with a 100-nucleotide pair periodicity. From chromatin, this pattern or the standard 200-nucleotide pair repeat can be produced depending on the ionic conditions. The results are interpreted by assuming different conformational states of the nuclear material, including condensed and extended forms.
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References
Elgin, S. C. R., and Weintraub, H., A. Rev. Biochem., 44, 725–774 (1975).
Lewin, B., Nature, 254, 651–653 (1975).
Felsenfeld, G., Nature, 257, 177–178 (1975).
Sollner-Webb, B., and Felsenfeld, G., Biochemistry, 14, 2915–2920 (1975).
Noll, M., Nucleic Acids Res., 1, 1573–1578 (1974).
Pfeiffer, W., Hörz, W., Igo-Kemenes, T., and Zachau, H. G., Nature, 258, 450–452 (1975).
Greil, W., Igo-Kemenes, T., and Zachau, H. G., Nucleic Acids Res., 3, 2633–2644 (1976).
Zachau, H. G., Altenburger, W., Greil, W., Hörz, W., and Igo-Kemenes, T., International Symposium on the Organization and Expression of Eukaryotic Genome (edit. by Bradbury, E. M., and Javaherian, K.), (Academic, New York, in press).
Woodcock, C. L. F., Sweetman, H. E., and Frado, L.-L., Expl Cell Res., 97, 111–119 (1976).
Gottesfeld, J. M., Murphy, R. F., and Bonner, J., Proc. natn. Acad. Sci. U.S.A., 72, 4404–4408 (1975).
Oosterhof, D. K., Hozier, J. C., and Rill, R. L., Proc. natn. Acad. Sci. U.S.A., 72, 633–637 (1975).
Bernardi, G., The Enzymes, third edn, 4 (edit. by Boyer, P. D.), 271–287 (Academic, New York, 1971).
Southern, E. M., J. molec. Biol., 94, 51–69 (1975).
Peacock, A. C., and Dingman, C. W., Biochemistry, 6, 1818–1827 (1967).
Fiers, W., et al., Proc. Tenth FEBS Meeting, 39 (edit. by Chaperville, F., and Grunberg-Manago, M.), 17–33 (North-Holland/American Elsevier, 1975).
Simpson, R. T., and Whitlock, J. P., Jr, Nucleic Acids Res., 3, 117–127 (1976).
McCarty, K. S. Jr, Vollmer, R. T., and McCarty, K. S., Analyt. Biochem., 61, 165–183 (1974).
Dingman, C. W., and Sporn, M. B., J. biol. Chem., 239, 3483–3492 (1964).
Bradbury, E. M., Carpenter, B. G., and Rattle, H. W. E., Nature, 241, 123–126 (1973).
Spadafora, C., and Geraci, G., Biochem. biophys. Res. Commun., 69, 291–295 (1976).
Weintraub, H., Proc. natn. Acad. Sci. U.S.A., 72, 1212–1216 (1975).
Varshavsky, A. J., and Georgiev, G. P., Molec. Biol. Reps 2, 255–262 (1975).
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Altenburger, W., Hörz, W. & Zachau, H. Nuclease cleavage of chromatin at 100-nucleotide pair intervals. Nature 264, 517–522 (1976). https://doi.org/10.1038/264517a0
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DOI: https://doi.org/10.1038/264517a0
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