Abstract
AN important problem pertinent to the mechanism of lymphocyte stimulation with a nonspecific mitogen is how long lymphocytes must be exposed to the mitogen to attain maximal stimulation. In the case of the stimulation of human and murine lymphocytes by concanavalin A (con A), the cells are irreversibly committed to stimulation if the lectin is (present on the cell membranes for some 18–20 h after the start of the culture1–3. Here we report studies on the kinetics of the stimulation of mouse splenic lymphocytes by con A. Our data indicate that two pulses of con A (0–3 h and 15–18 h after the start of the culture) can stimulate the cells to the same degree as the continuous exposure of the cells to con A for 18 h, suggesting that two signals are necessary for the con A stimulation of lymphocytes. We also show that the first signal is possibly given by the influx of Ca2+.
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TOYOSHIMA, S., IWATA, M. & OSAWA, T. Kinetics of lymphocyte stimulation by concanavalin A. Nature 264, 447–449 (1976). https://doi.org/10.1038/264447a0
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DOI: https://doi.org/10.1038/264447a0
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