Abstract
SKIN fibroblasts derived from patients with the autosomal recessive disease xeroderma pigmentosum are defective in excision repair of photodamage induced by short wavelength ultraviolet light1. In conjunction with the clinical observation that these patients are extremely prone to develop skin cancer2, it has been suggested that DNA repair plays a central role in the process of carcinogenesis. Although methodologies are available for investigating the molecular biology of mammalian DNA repair, studies of carcinogenesis induced by UVL have been limited largely to the use of the hairless mouse3–5. In a preliminary report, DiPaolo and Donovan6 reported the induction of transformation in primary hamster embryo cell cultures by ultraviolet light. Mondal and Heidelberger7 reported inducing malignant transformation in mouse 10T1/2 cells in vitro by exposure to ultraviolet light followed by treatment with tetradecanoyl phorbol acetate. We report here that ultraviolet light of 254-mm wavelength alone is sufficient to induce reproducible transformation in this cell line, and we present the dose–response relationship for this phenomenon.
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CHAN, G., LITTLE, J. Induction of oncogenic transformation in vitro by ultraviolet light. Nature 264, 442–444 (1976). https://doi.org/10.1038/264442a0
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DOI: https://doi.org/10.1038/264442a0
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