Abstract
The surface topography of concanavalin A (con A) bound to normal and transformed murine fibroblasts has been studied by a new technique involving fluorescence resonance energy transfer (RET). RET can provide a high resolution “map” of the distances separating con A–receptor complexes in single living cells. The distribution of con A is non-random in both normal and transformed cells, but sites are more closely approximated in the transformed. Approximation is induced by the con A but occurs at extremely slow rates indicating that the topography is not primarily determined by simple diffusion of complexes.
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References
Ozanne, B., and Sambrook, J., Nature new Biol., 232, 156–160 (1971).
Cline, M. J., and Livingston, D. C., Nature new Biol., 232, 155–156 (1971).
Collard, J. G., and Temmink, J. H. M., J. Cell Sci., 19, 21–32 (1975).
Nicolson, G. L., Nature new Biol., 233, 244–246 (1971).
Rosenblith, J. Z., Ukena, T. E., Yin, H. H., Berlin, R. D., and Karnovsky, M. J., Proc. natn. Acad. Sci. U.S.A., 70, 1625–1629 (1973).
Ukena, T. E., Borysenko, J. Z., Karnovsky, M. J., and Berlin, R. D., J. Cell Biol., 61, 70–82 (1974).
Förster, T. H., Disc. Faraday Soc., 27, 7–17 (1959).
Becker, J. S., Oliver, J. M., and Berlin, R. D., Nature, 254, 152–154 (1975).
Stryer, L., Science, 162, 526–533 (1968).
Steinberg, I. Z., and Katchalski, E., J. chem. Phys., 48, 2404–2410 (1968).
Dale, R. E., and Eisinger, J., Proc. natn. Acad. Sci. U.S.A., 73, 271–273 (1976).
Schiller, P. W., in Biochemical Fluorescence: Concepts, 1 (edit. by Chen, R. F., and Edelhoch, H.), 285–303 (Dekker, New York, 1975).
Shelanski, M. L., Gaskin, F., and Cantor, C. R., Proc. natn. Acad. Sci. U.S.A., 70, 765–768 (1973).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265–275 (1951).
Kinoshita, T., Iinuma, F., and Tsuji, A., Analyt. Biochem., 61, 632–637 (1974).
Oliver, J. M., Zurier, R. B., and Berlin, R. D., Nature, 253, 471–473 (1975).
Yguerabide, J., in Methods in Enzymology, 26, part C (edit. by Hirs, C. H. W., and Timasheff, S. N.), 498–578 (Academic, New York, 1972).
Inbar, M., and Sachs, L., Nature, 223, 710–712 (1969).
Noonan, K. D., and Burger, M. M., J. biol. Chem., 248, 4286–4292 (1973).
Smith, S. B., and Revel, J-P., Devl Biol., 27, 434–441 (1972).
Petris, S. de., Raff, M. C., and Mallucci, L., Nature new Biol., 244, 275–278 (1973).
Temmink, J. H. M., Collard, J. G., Spits, H., and Roos, E., Expl Cell Res., 92, 307–322 (1975).
Nicolson, G. L., Nature new Biol., 243, 218–220 (1973).
Zagyansky, Y., and Edidin, M., Biochim. biophys. Acta, 433, 209–214 (1976).
Jacobson, K., Wu, E., and Poste, G., Biochim. biophys. Acta, 433, 215–222 (1976).
Schlessinger, J. et al., Proc. natn. Acad. Sci. U.S.A., 73, 2409–2413 (1976).
Poo, M-M., and Cone, R. A., Nature, 247, 438–441 (1974).
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Fernandez, S., Berlin, R. Cell surface distribution of lectin receptors determined by resonance energy transfer. Nature 264, 411–415 (1976). https://doi.org/10.1038/264411a0
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DOI: https://doi.org/10.1038/264411a0
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