Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

X-ray diffraction studies of circular superhelical DNA at 300–10,000-Å resolution

Nature volume 264, pages 231–234 (1976)Cite this article

Abstract

X-ray diffraction studies on circular superhelical DNA from bacteriophage PM2 at a very low scattering angle show that it is possible to measure the superhelical scattering function of the molecule. The results suggest that in addition to the primary supercoil, a higher order of supercoiling of the DNA is present, an effect which can be interpreted with a simple analogue.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Helinski, D. R., and Clewell, D. B., A. Rev. Biochem., 40, 899 (1971).

    Article  CAS  Google Scholar 

  2. Espejo, R. J., Canelo, E. S., and Sinsheimer, R., Proc. natn. Acad. Sci. U.S.A., 93, 1164 (1969).

    Article  ADS  Google Scholar 

  3. Espejo, R. J., and Canelo, E. S., Virology, 34, 738 (1968).

    Article  CAS  Google Scholar 

  4. Salditt, M., Brownstein, S. N., Camerini-otero, R. D., and Franklin, R. M., Virology, 34, 738 (1968).

    Article  Google Scholar 

  5. Richardson, J. P., J. molec. Biol., 78, 703 (1973).

    Article  CAS  Google Scholar 

  6. Bonse, U., and Hart, M., Z. Physik., 189, 151 (1966).

    Article  ADS  CAS  Google Scholar 

  7. Kratky, O., Small Angle Scattering (edit. by Brumberger, H.), 13 (Gordon and Breach, New York, 1967).

    Google Scholar 

  8. Freifelder, D., J. molec. Biol., 60, 474 (1971).

    Article  Google Scholar 

  9. Freifelder, D., J. molec. Biol., 35, 303 (1968).

    Article  CAS  Google Scholar 

  10. Cochran, J., Crick, F. H. C., and Vand, V., Acta Crystullogr., 5, 581 (1952).

    Article  CAS  Google Scholar 

  11. Jones, R. C., Acta Crystallogr., 2, 252 (1949).

    Article  Google Scholar 

  12. Gray, A. B., Upholt, W. B., and Vinograd, J., J. molec. Biol., 62, 1 (1971).

    Article  CAS  Google Scholar 

  13. Upholt, W. B., Gray, H. B., and Vinograd, J., J. molec. Biol., 61, 21 (1971).

    Article  Google Scholar 

  14. Wang, J. H., J. molec. Biol., 43, 24 (1969).

    Google Scholar 

Download references

Author information

Author notes

  1. D. B. Fein

    Present address: New York State Department of Health, Division of Laboratories and Research, Empire State Plaza, Albany, New York, 12201

Authors and Affiliations

  1. Bell Laboratories, Murray Hill, New Jersey, 07974

    G. W. Brady & D. B. Fein

  2. Department of Chemistry, Syracuse University, Syracuse, New York, 13210

    H. Brumberger

  3. Department of Physics, State University of New York at Albany, Albany, New York, 12222

    D. B. Fein

Authors
  1. G. W. Brady
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. B. Fein
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Brumberger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brady, G., Fein, D. & Brumberger, H. X-ray diffraction studies of circular superhelical DNA at 300–10,000-Å resolution. Nature 264, 231–234 (1976). https://doi.org/10.1038/264231a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/264231a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing